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Practical course in recombinant microorganisms in biotechnology - MB140C63
Title: Praktikum z rekombinantních mikroorganizmů v biotechnologiích
Czech title: Praktikum z rekombinantních mikroorganizmů v biotechnologiích
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2020
Semester: summer
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:0/1, C [DS]
Capacity: 10
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: specialized
Note: enabled for web enrollment
Guarantor: RNDr. Mgr. Andrea Palyzová, Ph.D.
Teacher(s): RNDr. Helena Marešová, CSc.
RNDr. Mgr. Andrea Palyzová, Ph.D.
Co-requisite : MB140P63
Annotation -
Last update: RNDr. Mgr. Andrea Palyzová, Ph.D. (10.06.2016)
Practicum supplements the lecture "Recombinant microorganisms in biotechnology" about practical tasks advanced by cultivation of microorganisms in stirred bioreactor. Students will evaluate and deal with experiments individually. Practical course is aimed for graduate students of Microbiology and other disciplines. Practical course takes place in the Microbiological Institute of the Academy of Science.
Literature - Czech
Last update: RNDr. Irena Lichá, CSc. (13.03.2019)

Literatura:

In vitro cultivation of microorganisms. BIOTOL (Project); Open Universiteit (Heerlen, Netherlands); Thames Polytechnic. Oxford ; Boston : Butterworth-Heinemann, 1992.

Barredo, J.-L. (Ed.). 2005. Microbial Processes and Products. In: Methods in Biotechnology, volume 18, Humana Press. https://doi.org/10.1385/1592598471

Glick B.R., Pasternak J.J., 2002. Principles and Applications of Recombinant DNA. In: Molecular Biotechnology. ASM Press.

Filloux, A., Ramos, J.-L. (Eds.). 2014. Pseudomonas Methods and Protocols. In: Methods in Molecular Biology, volume 1149, Humana Press, https://doi.org/10.1007/978-1-4939-0473-0.

Stanbury P.F., Whitaker A., Hall S.J., 2016. Principles of Fermentation Technology. https://doi.org/10.1016/C2013-0-00186-7
Requirements to the exam -
Last update: RNDr. Michaela Schierová, Ph.D. (24.10.2019)

To get the credit, active participation in a practical course is required.
Syllabus -
Last update: RNDr. Michaela Schierová, Ph.D. (29.10.2019)

Experimental part of cultivation practice focused on production of bacterial strains in continuously stirred bioreactor (CSTR)

 

1) Single batch fermentation in a stirred bioreactor - example of microbial product production in a closed 
system, where all nutrients will be consumed during cultivation, resulting in accumulation of biomass and 
extracellular product.
2) Fed-fermentation in a stirred bioreactor - example of cultivation of recombinant 
high-expression bacterial system, where nutrients will be controlled in the course of cultivation, 
flow rate of substrate stock solution is controlled by dissolved oxygen concentration (pO2)
 
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