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Electrophoretic methods in the analysis of biologically active compounds - GDBM03

Title:	Elektroforetické metody v analýze biologicky aktivních látek
Guaranteed by:	Department of Analytical Chemistry (16-16140)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2019 to 2023
Semester:	both
Points:	0
E-Credits:	0
Examination process:
Hours per week, examination:	0/0, Ex [HT]
Capacity:	winter:unknown / unknown (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Note:	course is intended for doctoral students only enabled for web enrollment you can enroll for the course in winter and in summer semester

Guarantor:	prof. RNDr. Juraj Ševčík, Ph.D.
Interchangeability :	GDZCH03
Is interchangeable with:	GDZCH03

Examination dates WS schedule SS schedule

Annotation -

Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (28.02.2018)

Development trends in electrophoretic methods, miniaturization, chip electrophoresis. Electrophoresis in proteomic studies. Non-aqueous capillary electrophoresis and its applications. Selected applications of capillary electrophoresis - separation of peptides and proteins, chiral separation. On-line "stacking" techniques in capillary electrophoresis for the analysis of biological materials. Micellar electrokinetic chromatography. Connection of capillary electrophoresis with advanced detection techniques and mass spectrometry.

Literature - Czech

Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (28.02.2018)

Povinná:

Garcia, Carlos D. (ed.). Capillary electrophoresis and microchip capillary electrophoresis principles, applications, and limitations. Hoboken, New Jersey: Wiley, 2013, 394 s. ISBN 978-0-470-57217-7.

Nicola Volpi . Capillary Electrophoresis of Biomolecules . London: Humana Press , 2013, s. ISBN 978-1-62703-295-7.

Doporučená:

Web of Knowledge [online]. Dostupné z: http://apps.webofknowledge.com

sciencediretc [online]. Dostupné z: https://www.sciencedirect.com

Doporučená:

M.G. Khaledi . High Performance Capillary Electrophoresis – Theory, Techniques and Applications, . Amsterdam : Willey, 1998, s. ISBN .

Doporučená:

. . In Electrophoresis, Journal of Separation Sciences, Journal of Chromatography A. (): -.

Requirements to the exam -

Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (28.02.2018)

Theoretical aspects of electromigration techniques.

Detection techniques in capillary electrophoresis (CE).

CE in drug and macromolecular analyzes, proteomic analyzes.

Modern trends in electromigration techniques.

The CE for on-line pre-concentration of trace substances.

Trends in micellar electrophoresis and capillary electrochromatography.

Trends in CE coupling with Mass Detection for Biomolecular Analysis.