Stem Cells and Regenerative Medicine - FV045

• Title: Stem Cells and Regenerative Medicine
• Guaranteed by: Department of Histology and Embryology (15-140)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2022
• Semester: summer
• Points: 0
• E-Credits: 2
• Examination process: summer s.:
• Hours per week, examination: summer s.:25/7, C [HT]
• Capacity: 35
• Min. number of students: 5
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: prof. MUDr. Jaroslav Mokrý, Ph.D.; MUDr. Tomáš Soukup, Ph.D.
• Comes under: General medicine - ELECTIVE subjects
• Pre-requisite : {Malá skupina pro FV045}
• Incompatibility : FG50019
• Interchangeability : FG50019
• Is incompatible with: FG50019
• Is interchangeable with: FG50019

Annotation

Last update: Markéta Ulrichová (17.01.2024)

In lectures, students gain knowledge in a progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering and cell therapy. In the course of practical classes, students become familiar with detection methods and basic procedures in the tissue culture laboratory.

Entry requirements

Last update: Markéta Ulrichová (17.01.2024)

Students who have passed histology, embryology and biology are eligible to register.

Aim of the course

Last update: Markéta Ulrichová (17.01.2024)

Outcomes:

1. the student acquires theoretical knowledge ofa progressively developing field of stem cell biology and regenerative medicine incl. closely related areas of tissue engineering, IVF and cell therapy.
2. in the course of practical classes, students become familiar with SCs isolation procedures, detection methods and basic techniques in the tissue culture laboratory.

Requirements to the exam

Last update: Markéta Ulrichová (17.01.2024)

Conditions for obtaining credits:

Presence in lectures and laboratory classes. Absences may be substituted only by writing an assay on the topic you missed. Elaboration of protocol for cell viability.

Syllabus

Last update: Markéta Ulrichová (17.01.2024)

Lecturers:

• prof. MUDr. Stanislav Filip, Ph.D.
• prof. MUDr. Jaroslav Mokrý, Ph.D.
• prof. MUDr. Pavel Žák, Ph.D.
• MUDr. Tomáš Soukup, Ph.D.
• doc. MUDr. Jakub Suchánek, Ph.D.

Lectures:

1. Introduction. Regenerative medicine, regeneration, reparation. Cell therapy. Stem and progenitor cells - basic terms, characterization, biological behaviour, differentiation potential. Stem cell plasticity. Telomeres, telomerase and ageing of stem cells. Ethics and moral aspects of stem cell research.
2. Pluripotent stem cells, ES cells, iPS cells. Epithelial stem cells. Tumour stem cells. Muscle-derived stem cells; muscle regeneration.
3. Neural stem cells and their niche. Regeneration of the nervous tissue. Spinal cord injury.
4. Endothelial progenitor cells. Haematopoietic stem cells. Haematological applications, bone marrow transplantation.
5. Mesenchymal stem cells. Dental pulp stem cells. Separation techniques. Cellular interactions. Growth factors.
6. Scaffolds (2D, 3D) - natural, artificial. Encapsulation. Nanotechnology. Cellular migration. Cell differentiation. Cell transplantation. Tissue engineering of bones and cartilages. Orthopaedic applications.

Practical courses:

1. Tissue engineering. Laboratory facilities and basic procedures in tissue culture laboratory. Safety aspects, risk assessment. Preparation of buffers and media. Phase contrast microscopy.
2. Cultivation techniques. Isolation of cells from human and animal tissues. Enzymatic tissue dissociation. Cell quantification. Cryoconservation. Mathematics in the laboratory - number of population doublings, doubling time, kinetics.
3. Cell isolation. Primary cultures. Subculturing. Cultivation surfaces. Basic biological characteristics. Cell separation techniques. Gradient centrifugation (Ficoll, Percoll). Immunomagnetic separation.
4. Cultivation protocols. Stem cell differentiation in vitro. Extracellular matrix analysis.
5. Visit of tissue culture laboratory. Cell viability. Preparation 

Literature

Last update: Markéta Ulrichová (17.01.2024)

Recommended literature:

1. Alberts et al. Molecular Biology of the Cell, W. W. Norton & Company; Sixth edition (November 18, 2014), ISBN 9780815344322
2. Atala et al. Handbook of Stem Cells, Academic Press; 2 edition (October 8, 2012), ISBN 9780123859426
3. Wingard et al. Hematopoietic Stem Cell Transplantation: A Handbook for Clinicians, 2nd edition, American Association of Blood Banks (AABB); 2nd edition (January 28, 2015), ISBN 1563958767
4. Clark et al. The Molecular and Cellular Biology of Wound Repair, Springer; 1988 edition (October 24, 2012), ISBN 146135725X
5. Zhou et al. Mammalian Cell Cultures for Biologics Manufacturing (Advances in Biochemical Engineering/Biotechnology), Springer; 2014 edition (January 16, 2014), ISBN 364254049X
6. Bronzino et al. The Biomedical Engineering Handbook: Four Volume Set, CRC Press; 4 edition (April 10, 2015), ISBN 1439825335
7. Ratner et al. Biomaterials Science: An Introduction to Materials in Medicine, Academic Press; 3 edition (November 8, 2012), ISBN 9780123746269
8. Frank H. Stephenson Calculations for Molecular Biology and Biotechnology, Academic Press, Elsevier Science 2003, ISBN 0-12-665751-3