Bacteriology: Gram positive and G negative bacteria, basic characteristic, pathogenicity, therapy and prevention of bacterial infections. Antibiotics: mechanisms of action, resistance, susceptibility testing, rules of ATB therapy and rules on the use. Virology: RNA and DNA viruses, Prions, basic characteristics. The antiviral therapy, the prevention of viral diseases. Mycology: basic characteristics of pathogenic fungi, local and systemic infections, opportunistic infections, mycoses and antifungal therapy. Parasitology: Protozoa, worms, basic characteristics, cycles in nature, in animals and in human population, pathogenicity. Arthropods: causative agents of diseases and vectors. Therapy and prevention of parasitic infections. Basics of clinical microbiology. Nosocomial infections and diseases. Vaccines and principles of immunization. Laboratory methods in microbiology.
Requirements to the exam
Last update: Eva Dyntarová (28.01.2016)
Credit
Credit will be given to students who:
fulfill the attendance requirements (1 legitimate absence per term)
have their written protocols in order
have an average mark of tests up to 3,0
Syllabus - Czech
Last update: Eva Dyntarová (28.01.2016)
Lecturers
Assoc. Prof. Vladimir Buchta, M.Sc., Ph.D.
Zuzana Cermakova, D.V.M., Ph.D.
Vlasta Stepanova, M.D., Ph.D.
No. Theme Lecturers Duration
Introduction to medical microbiology. (Assoc.Prof. Žemličková)
Practical courses & seminars Lecturers
Assoc. Prof. Vladimir Buchta, M.Sc., Ph.D.
Zuzana Cermakova, D.V.M., Ph.D.
Vlasta Stepanova, M.D., Ph.D.
Practical courses
Microbiology Safety, devices, equipment, workflow in microbiology lab. Students are introduced into safety rules in microbiological laboratory and teaching organizations. Attention is paid to the issue of disinfection and sterilization. Hand hygiene.
Preanalytical phase. Collection, transport, and maintenance of biological materials. Demonstration of correct procedures of collection and transport of clinically significant samples.
Basic principles of microbiological diagnostics. Direct tests Part 1 (microscopy). Description of bacterial cell, staining, microscopy technique
Basic principles of microbiological diagnostics. Direct tests Part 2 (cultivation, biochemistry, serology). Cultivation, culture media, length of cultivation, other factors. MALDI-TOF MS.
Establishment of antibiotic susceptibility Antibiotic susceptibility methods, qualitative/ quantitative examination antibiotic susceptibility; break-point, interpretation of test results.
Basic principles of microbiological diagnostics. Indirect tests. Microbiological methods in virology. Serological methods - principles (agglutination, precipitation, complement-fixation, ELISA). Virology - direct examination.
Basic principles of microbiological diagnostics. Microbiological methods in mycology and parasitology. Molecular-biological methods. Microscopy, cultivation. Principle of PCR, the pros and cons. Indication of the methods in clinical practice.Credit test.
Postanalytical phase. Interpretation of microbiological methods, human microbiota; primary and opportunistic pathogens. Credit.
Notes: Actual informations about microbiology lectures and practical classes you can find on the board on the first floor of the department of microbiology (the building No. 17, Faculty Hospital).
Literature
Last update: Eva Dyntarová (28.01.2016)
Compulsory literature
W.A. Strohl, H. Rouse, B.D. Fischer Microbiology, Lippincott Williams a Wilkins, 2001,ISBN 0-397-51568-5
W. Levinson, E. Jawetz Medical Microbiology and Immunology. 4th ed.,Appleton and Lange, Stamford, USA, 1996. ISBN 0-8385-6225-6 (and newer editions)
Recommend literature
G. F. Brooks et al. Medical Microbiology. 3th ed., Lange, 2004. ISBN 0-07-141207-7
C. A. Mims et.al. Medical Microbiology. Mosby, 1993. ISBN 0-397-44631-4 (and newer editions)
