Biochemistry II - FG10014

• Title: Biochemistry II
• Guaranteed by: Department of Medical Biochemistry (15-180)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2018 to 2019
• Semester: summer
• Points: 0
• E-Credits: 10
• Examination process: summer s.:
• Hours per week, examination: summer s.:48/84, C+Ex [HS]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: deregister from the exam date if a requisite was not fulfilled
• Guarantor: prof. MUDr. Martina Řezáčová, Ph.D.
• Co-requisite : FG10013
• Incompatibility : FV10012
• Interchangeability : FA0104036, FV10012
• Is incompatible with: FV10012
• Is pre-requisite for: FG50032
• Is interchangeable with: FV10012
• In complex pre-requisite: FG10036, FG10038, FG10039, FG10040, FG10041, FG10042, FG10043, FG10044, FG10045, FG10046, FG10047, FG10048, FG10049, FG10075, FG10076, FG10077, FG10078, FG10079, FG10080, FG10083

Annotation

Last update: doc. MUDr. Alena Stoklasová, CSc. (25.01.2021)

Biochemistry: Overview of intermediary metabolism, biological oxidation and bioenergetics, the citric acid cycle, metabolism of carbohydrates, lipids, eicosanoids, steroids and lipoproteins, ketogenesis, metabolism of proteins and amino acids, ammonia formation and its removal, metabolism of porphyrins and bile pigments. Metabolism of tissues: blood, water and ions metabolism, acid-base balance, haemostasis and blood clotting, contractile and structural proteins, mechanism of muscle contraction, metabolism of connective tissue, metabolism of adipose tissue, metabolism of neurotransmitters, extracellular and intracellular communication, hormones - their synthesis, degradation and effects, xenobiochemistry and immunochemistry.

Requirements to the exam

Last update: doc. MUDr. Alena Stoklasová, CSc. (22.01.2021)

Credit:

a) Attendance at laboratory classes and seminars

b) Complete protocols; must  be presented at the last class in the summer term

c) Result of all examinations: at least 3.00 (written tests and oral examinations; written tests are obligatory and any missing test must be substituted)

d) Power point presentation at a given topic; passing result in four of minitests
 

Examination:

The examination consists of two parts - multiple choice questionnaire test and oral examination - and it is conditioned with having both credits. Written test:  at least 11 points are necessary for oral part of examination (32 points max.). The students who fail to satisfy the requirements of the examination are re-examined orally (two possibilities).

 

Syllabus

Last update: doc. MUDr. Alena Stoklasová, CSc. (25.01.2021)

 Lectures

1. Carbohydrates. Classification, Structure, Properties, Chemical Reactions. (Řezáčová 2)
2. Metabolism of Glucose, Fructose, Galactose, Uronic Acids; Glycosaminoglacans ang Glycoproteins. (Řezáčová 4) 
3. Arteficial Sweeteners. (Řezáčová 1) 
4. Lipids and Steroids. Classification, Structure, Properties, Chemical Reactions. (Stoklasová 1) 
5. Metabolism of Lipids and Steroids - Digestion, Resorption and Transport.  Biosynthesis and Degradation of Saturated and Unsaturated Fatty Acids. Ketogenesis. Eicosanoids. Metabolism of Acylglycerols and Sphingolipids. Metabolism of Cholesterol. Biosynthesis of Bile Acids and Steroid Hormones. Lipoproteins. (Stoklasová 4) 
6. Lipoproteins. (Stoklasová 1) 
7. Metabolism of Proteins and Amino Acids -Digestion, Resorption and Transport. Transamination, Oxidative Deamination and Decarboxylation of Amino Acids. (Muthná 2) 
8. Catabolism of the Carbon Skeleton of Amino Acids. Ammonia Formation and its Removal. Biogenic Amines. (Stoklasová 5) 
9. Metabolism of Porphyrins and Bile Pigments. Biosynthesis of Haeme and Haemoglobin and their Catabolism. Jaundice. (Řezáčová 2) 
10. Components of the Blood. Water and Ions Metabolism. Acid-Base Balance. The Lungs and Kidneys in Acid-Base Balance. Disorders of Acid-Base Balance.  (Řezáčová 2) 
11. Metabolism of Erythrocytes. Haemostasis and Blood Clotting. (Řezáčová 2) 
12. Metabolism of Kidneys. (Řezáčová 1) 
13. Metabolism of Muscles. Contractile and Structural Proteins. Mechanism of Muscle Contraction and its Energy Supply. (Mrkvicová 2) 
14. Metabolism of Connective Tissue. Connective Tissue Proteins, Proteoglycans. (Mrkvicová 3)
15. Metabolism of Bones and Teeth. Process of Mineralization. (Stoklasova 1)
16. Digestive Tract and Liver. Importance in Metabolism. (Řezáčová 2) 
17. Metabolism of Adipose Tissue. (Řezáčová 1) 
18. Metabolism of Nerve System (Řezáčová 2) 
19. Extracellular and Intracellular Communication. Second Messengers, Protein Kinases. (Řezáčová 2)
20. Hormones and their Action. Synthesis, Degradation, Effects: Hormones of Hypothalamus, Pituitary Gland, Thyroideal Gland, Parathyroideal Gland, GIT, Pancreas and Adrenal Medulla. (Cerman 4)      
21. The Comparison of the Metabolic Pathways in Liver, Muscles, Adipose Tiissue and CNS. (Stoklasová 2) 
22. Introduction to Xenobiochemistry. Two Phases of Biotransformation. (Hroch 2) 
23. Introduction to Immunochemistry. (Stulík 2) 

 
Laboratory classes & seminars

Lecturers

• Prof. Martina Řezáčová, M.D., Ph.D.
• Assoc. Prof. Ing. Miloš Hroch, Ph.D.
• Assoc. Prof. Alena Stoklasová, M.D., Ph.D.
• Dr, Ivana Brabcová, Ph.D.
• Dr. Radim Havelek, Ph.D.
• Dr. Renata Kohlerová, Ph.D.
• Dr. Alena Mrkvicová, Ph.D.
• Dr. Darina Muthná, Ph.D.
• Dr. Darja Koutová
• Dr. Ondřej Martinec
• Dr. Lucie Melounková
• Dr. Eva Peterová
• Dr. Martin Uher

 
Seminars

1. Carbohydrates - Classification, Structure, Properties, Digestion, Absorption, Transport. Metabolism of glucosse.
2. Metabolism of Fructose, Galactose, Uronic Acids; Glycosaminoglacans ang Glycoproteins. Test.
3. Metabolism of Lipids - Digestion, Absorption, Transport. Metabolism of Fatty Acids, Triacylglycerol, Phospho-, Sphingo- and Glycolipids. Ketone Bodies. Test.
4. Metabolism of Steroids - Classification, Structure, Properties. Metabolism of Cholesterol, Bile Acids and Steroid Hormones. Test.
5. Metabolism of Lipoproteins, Hyperlipoproteinaemias. Atherosclerosis. Presentations. Minitest.
6. Proteins - Digestion, Absorption, Transport. Metabolism of Ammonia, Biosynthesis of Urea.
7. Metabolism of Amino Acids. Hormones and Neurotransmitters derived from amoíno Acids Test.
8. Metabolism of Porphyrins and Bile Pigments. Metabolism of Erythrocytes and Leukocytes.Presentations.
9. Acid-Base Balance. Minerals and Water in the Body and their Regulation.
10. The Blood, Blood Clotting. Importance of Eicosanoids. Presentations. Minitest.
11. Metabolism of Contractile Tissues. Presentations. Minitest.
12. Metabolism of Connective Tissues. Presentations. Minitest.
13. Metabolism of Bones and Teeth and their Hormonal Regulation. Test.
14. Extracellular and Intracellular Communication. Receptors. Metabolism of Nerve System. Biochemistry of Vision. Presentations. Minitest.
15. Structure and Properties of Hormones of Hypothalamus, Pituitary Gland, Thyroideal Gland, Adrenal Medulla and Pankreas. Regulatory Peptides in GIT.     
16. Relationship between Metabolism of Carbohydrates, Lipids and Proteins in some Tissues and their Regulation.  Metabolism of Adipose Tissue. Presentation. Test.
17. Metabolism of Xenobiotics. Presentations.    

Laboratory classes

1. Intorduction to the Chemical Methods in Clinical Laboratory.
2. Processing of Samples.
3. Glucose Concentration in the Serum. Presentation.
4. Protein Concentration in the Serum. Separation of the Serum Proteins by Electrophoresis.
5. Concentrations of Urea in the Serum. Concentration of Creatinine in the Serum and Urine.
6. Concentrations of Total and HDL-Cholesterol in the Serum.
7. Concentrations of Calcium and Phosphates in the Serum. Presentation.
8. Liver Diseases. Catalytic Concentrations of Alanine Aminotransferase and Alkaline Phosphatase in the Serum. Presentation.
9. Qualitative Analysis of the Urine. Proteins, Glucose, Blood, Acetone and Ehrlich-positive Substances. Presentation.

Notes: Attendance at laboratory classes and seminars is COMPULSORY and a register is kept. For unavoidable absences (e. g. illness) a written note must be given to doc. Stoklasová immediately on your return.

         

 

Literature

Last update: doc. MUDr. Alena Stoklasová, CSc. (25.01.2021)

Compulsory literature

1. R. Y. Ouellette Introduction to General, Organic and Biological Chemistry. 3rd ed., Mac Millan Publ.Company, New York, 1992. ISBN 0-02-390245-0 (or the latest edition available)
2. R. K. Murray et al. Harper's Biochemistry. 24th ed., Appleton and Lange, Stamford, USA, 1996.ISBN 0-8385-3612-3. (or the latest edition available)
3. J.W. Baynes et M. H. Dominiczak Medical Biochemistry. 4th ed., Elsevier 2014. ISBN 978-1-4557-4580-7
    

Recommend literature

1. P. C. Champe and R.A.Harvey Biochemistry. Lippincott Co, 1987. ISBN 0-397-50801-8 (or the latest edition available)