Biology II - FG10012

• Title: Biologie II
• Guaranteed by: Department of Medical Biology and Genetics (15-120)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2011 to 2015
• Semester: winter
• Points: 0
• E-Credits: 5
• Examination process: winter s.:
• Hours per week, examination: winter s.:26/39, C+Ex [HS]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Note: deregister from the exam date if a requisite was not fulfilled
• Guarantor: prof. MUDr. RNDr. Miroslav Červinka, CSc.
• Incompatibility : FV10010
• Interchangeability : FA0103035, FA0103045, FV10010
• Is incompatible with: FV10010
• Is interchangeable with: FV10010
• In complex pre-requisite: FG10076, FG10077, FG10078, FG10079, FG10080

Annotation

Last update: Marcela Laláková (21.09.2016)

Human karyotype. Structural and numerical aberration of autosomes. Sexual reproduction, meiosis, abnormalities of gonosomes. X-linked genes, X chromosome inactivation. Genetic manipulations - recombination of DNA, PCR, DNA sequencing. DNA diagnostics. Production of recombinant molecules. Gene therapy. Genetics of multicellular organisms, Mendel's law, Morgan's law. Clinical genetics - inborn errors of metabolism, prenatal diagnosis, genetic counselling. Human genetics - pedigree analysis, siblings, genomics Gene control of immunity, antibodies, monoclonal antibodies. Immunologic tolerance, transplantation immunity. Population genetics - Hardy-Weinberg law - its application and consequences. Evolution of species, evolution of Homo sapiens.

Requirements to the exam

Last update: Marcela Laláková (21.09.2016)

Requirements for credit

a)    Attendance + active participation

• Attendance at practical classes is mandatory. Each student is allowed one absence (medically excused) from one practical class.
• Practicals begin at 11.10 a.m. and students are required to be in their seat. If the student is not present at that time, he will be marked absent.
• Students must be prepared for the class.  

b)    Report, karyotype, presentation, manual

Studentsareobligedtohandintheirownkaryotypealongwithareferencephotograph.Thedeadlineis November 22^th 2013.

• Students are also obliged to preparean abstract of their oral presentation and submit this abstract via email for checkout.The topics will be specified at the beginning of the semester. The deadline is December 6^th 2013 . In case of the inappropriate presentation topic, we reserve the right to reject it and ask for the refinement or topic change. The full text version of presented topics should be submitted by e-mail before December 20^th 2012.
• Manualpresentedduringcreditsessionmusthaveanappropriateexternalform;i.e.beclean,intactasithasstatusofofficialdocument.

c)    Tests

There is one final test which must be passed. Passing grade is 75 %. In case of the test failure, the student has two more chances to pass written make-up tests.

d)    Credit
The credit in this term consists of:

Final test with minimal scoring grade of 75 %

• Completedmanualincludingdrawingsandanswerstoquestions (in case of any manual damage or loss, the student has to acquire a new one from the department against financial compensation).
• Preparedkaryotype
• Successfully presented topic (evaluation of presentation will include the topic itself as well as the quality of presentation)

Appointmentsforwintercreditarescheduledtowinter examination period (January 20^st 2014 - February 16^th 2014), summer examination period (June 2^rd 2014 - June 29 ^th 2014) andduring September (1. - 26. 9. 2014),each week on Thursdays from 2 to 3pm only.

Generally, in case of failure in credit (final test), the next possible chance is after one week!!!!

The students who seek the exemption from practical classes in medical biology and genetics have to submit their request before the start of practical classes. Their request must be accompanied by a detailed syllabus of biology course they had completed which will be evaluated by the head of the department of medical biology and genetics.

e)    Examination

• Before signing for the final examination each student must have two credits, one credit in the summer term of the first year and the second one in the winter term of the second year.
• Cheating during any form of biology exam (credit test and oral examination) including preparatory period is not tolerated! Use of any electronic devices (cell phones, tablets, notebooks, phablets etc.), unauthorized materials or communication with other students in these instances is strictly prohibited! After selecting final exam questions it is not allowed to leave the room and when necessary an escort will be provided. In case of failure to comply with these rules, student will be classified "failed".

Syllabus

Last update: Marcela Laláková (21.09.2016)

Lecturers

• Prof. Dr. Miroslav Cervinka, M.D., Ph.D.
• Prof. Dr. Emil Rudolf, Ph.D.

 

October 7

The mitochondrion, production of ATP, mitochondrial DNA.

The cytoskeleton (microtubules, microfilaments, intermediate filaments), cell motility, movement of organels, cellular motors.

         

October 14

Cell growth and cell division, cell cycle, mitosis.

Cell death, apoptosis.

        

October 21

Gene, genetic code.

Mutations, reparation of damaged DNA, screenings for mutagens.

Chromosomes, human karyotype. Structural and numerical aberrations of autosomes.

               

November 4

Sexual reproduction, meiosis, abnormalities of gonosomes

X-linked genes, X chromosome inactivation.

               

November 11

Genetic manipulations - basic methods and principles.

Recombination of DNA, PCR, cDNA.

               

November 18

DNA sequencing, genomics.

                

November 25

DNA diagnostics.

Gene therapy.

              

December 2

Genetics of multicellular organisms, Mendel’s law, Morgan’s law, pedigree analysis.

               

December 9

Twins in genetic research, cytogenetics, prenatal diagnosis.

Population genetics.

               

December 16

Metabolic disorders.

Clinical genetic.

 

 
Practical courses

 Lecturers

• Prof. Dr. Emil Rudolf, Ph.D.
• Dr. Vera Kralova, Ph.D.

 

7., 9. 10.              

1.            The cell cycle and cell division

1.1          Mitosis in permanent mounts of plant and animal cells

1.2          Mitosis in cells of cell cultures

1.3          Determination of the mitotic index and particular phases of mitosis

                              

14., 16. 10.         

2. A        Karyotyping using human cells (1st subgroup)

2. B        The summer term DNA samples- electrophoresis (2nd subgroup)

                              

21., 23. 10.         

3. A        The summer term DNA samples- electrophoresis (1st subgroup)

3. B        Karyotyping using human cells (2nd  subgroup)

                              

30. 10.  

4.            Eukaryotic chromosomes and meiosis

4.1          Meiosis in cells of locust

 

4., 6. 11.              

5.            Cytogenetic tests and their use in clinical genetics

5.1          Chromosome identification and classification (banding, SCE, FISH and other techniques)

                                              

               

11., 13. 11.         

6.            Sex chromatin

6.1          Sex chromatin in leukocyte smear

6.2          Evaluation of the prepared karyotypes

                              

18., 20. 11.         

7.            Mendelian genetics-pedigrees (workshop I)

7.1          Pedigree analysis, symbols and rules applied

                              

25., 27.11.          

8.            Molecular biology techniques in human genetics

               Biological typing in man (workshop II)

8.1          DNA diagnostics

                              

2., 4. 12.              

9.            Tumor biology I.

9.1          Normal and tumor cell in vitro and in vivo

9.2          Karyotypes of normal and tumor cells

                              

9., 11. 12.           

10.         Tumor biology II. (seminar)

10.1       Cell malignant transformation - mutagenesis and carcinogenesis

                              

16., 18. 12.         

11.         Population genetics (seminar)

11.1       Introductory concepts - Hardy-Weinberg law

                              

6., 8.1. 2015       

12.         Advances in molecular biology in 2014

12.1       Credit test

12.2       Students presentation I

                              

13., 15. 1.

13.         CREDIT

13.1       Students presentation II

                              

 

 

 

Literature

Last update: Marcela Laláková (21.09.2016)

Compulsory literature

1. B. Alberts et al. Essential Cell Biology. An introduction to the molecular biology of the cell. Garland Publishing, Inc. New York & London, 1998. ISBN 0-8153-2971-7
2. M. Connor et al. Essential Medical Genetics. 5th ed., Blackwell Science Ltd., 1997. ISBN 0-632-04158-7
3. Nussbaum, McInnes, Willard Thompson and Thompson Genetics in Medicine. 6th ed. revides reprint, Saunders, 2004. ISBN 0-7216-0244-4

 

Lectures and recommended literatur

Handouts and other materials provided by the Department, homepage of Department of Medical Biology and Genetics.