Human karyotype. Structural and numerical aberration of autosomes. Sexual reproduction, meiosis, abnormalities of gonosomes. X-linked genes, X chromosome inactivation. Genetic manipulations - recombination of DNA, PCR, DNA sequencing. DNA diagnostics. Production of recombinant molecules. Gene therapy. Genetics of multicellular organisms, Mendel´s law, Morgan´s law. Clinical genetics - inborn errors of metabolism, prenatal diagnosis, genetic counselling. Human genetics - pedigree analysis, siblings, genomics. Gene control of immunity, antibodies, monoclonal antibodies. Immunologic tolerance, transplantation immunity. Population genetics - Hardy-Weinberg law - its application and consequences.
Last update: Marcela Laláková (08.02.2019)
Karyotyp - poruchy počtu a struktury autosomů. Sexuální reprodukce - meiosa, inaktivace X chromosomu, změny pohlavních chromosomů. Genové manipulace - rekombinace DNA, vektory, PCR, určení sekvence DNA, klonování DNA. DNA diagnostika, produkce léčiv, genová terapie. Mendlova pravidla, Morganovy zákony. Klinická genetika, geneticky podmíněné metabolické poruchy.Metody lidské genetiky - studium rodokmenů, studium dvojčat, cytogenetika, DNA diagnostika. Imunologie,genová kontrola imunity, monoklonální protilátky. Transplantační imunita, imunologická tolerance. Genetika populací - Hardy-Weinbergův zákon, jeho aplikace a důsledky.
Requirements to the exam
Last update: Marcela Laláková (07.02.2019)
CREDIT:
· Final test must be passed. Passinggradeis75%.
· The credit in this term consists of:
° Final test with minimal scoring grade of 75 %
° Completed workbook including drawings (in case of any manual damage or loss, the student has to acquire a new one from the department against financial compensation).
Credit appointments (credit record) are limited to each week on Thursdays from 2 to 3pm only with exception of summer holidays (July and August).
Each student has three attempts. Failed credit test may be repeated two times (all written) at agreed dates. Generally, in case of failure in credit, the next possible chance is after one week!!!! The credit record has to be obtained 24h before the final exam date at the latest!
Final note – not fulfilling any of the above mentioned requirements (i.e. scoring grade in the final test below 75% including retake tests and incomplete workbook) will result in failure and credit will not be given!!!
Cheating during any form of biology exam (credit test and oral examination) including preparatory period is not tolerated! Use of any electronic devices (cell phones, tablets, notebooks, phablets etc.), unauthorized materials or communication with other students in these instances is strictly prohibited! After selecting final exam questions it is not allowed to leave the room and when necessary an escort will be provided. In case of failure to comply with these rules, student will be classified “failed”.
Syllabus
Last update: Marcela Laláková (07.02.2019)
Lectures
Lecturer: RNDr. Věra Králová, Ph.D.
Human chromosomes, chromosome abnormalities. Mendel´s laws, Morgan´s laws, gene linkage.
Inheritance of quantitative traits, multifactorial inheritance, heritability of traits. Immunogenetics.
Genomics. Pharmacogenetics. Recombinant DNA technologies, gene manipulations.
The prevention and treatment of genetic diseases. Population genetics.
Practical courses
Lecturers
Prof. Dr. Emil Rudolf, Ph.D.
Dr. Veronika Skarková, Ph.D.
Practical courses
Meiosis and gene linkage Polytene chromosomes in Drosophila Meiosis in cells of locust
Karyotyping of human cells
Cytogenetic tests and sex chromatin Chromosome classification and identification (banding and newer techniques) Karyotype of human cells Sex chromatin in leukocyte cells
