DNA/RNA Sequencing Technology and Bioinformatics - EDPLM0021

• Title: DNA/RNA Sequencing Technology and Bioinformatics
• Guaranteed by: Department of science and research (14-260)
• Faculty: Faculty of Medicine in Pilsen
• Actual: from 2023
• Semester: winter
• Points: 0
• E-Credits: 0
• Examination process: winter s.:
• Hours per week, examination: winter s.:6/3, C [HS]
• Capacity: unknown / unknown (unknown)
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Guarantor: prof. Ing. Jaroslav Hrabák, Ph.D.

Annotation

Last update: Mgr. Barbora Stachová (06.05.2024)

The course will lead to the development of knowledge in modern sequencing techniques, i.e. whole genome
sequencing on different
platforms. It will also cover RNA sequencing for the determination of protein expression.

Bioinformatics approaches are an integral part of data analysis. Credit is given for participation.

Literature

Last update: Mgr. Barbora Stachová (06.05.2024)

Patrick Murray Ken Rosenthal Michael Pfaller: Medical Microbiology, 2016, ISBN: 9780323299565

Fournier PE, Drancourt M, Colson P, Rolain JM, La Scola B, Raoult D. Modern clinical microbiology: new challenges and solutions. Nat Rev Microbiol. 2013 Aug;11(8):574-85.

Syllabus

Last update: Mgr. Barbora Stachová (06.05.2024)

Nucleic acid sequencing techniques affect many medical disciplines, not least microbiology. This course will summarize the possibilities of DNA and RNA sequencing, ranging from first-generation sequencing to sequencing of long DNA fragments. Students will also have the opportunity to learn about these techniques in a practical way. Emphasis will be placed on whole genome sequencing of the microbial genome (bacteria, viruses, micromycetes), including analysis of mobile genetic elements (especially plasmids). Students will be introduced to the possibilities of bioinformatic data analysis, including various software tools.