Subjects

Your browser does not support JavaScript, or its support is disabled. Some features may not be available.

Biochemistry I - EA0902400

Title:	Biochemie I
Guaranteed by:	Ústav lékařské chemie a biochemie (14-70)
Faculty:	Faculty of Medicine in Pilsen
Actual:	from 2020
Semester:	summer
Points:	4
E-Credits:	4
Examination process:	summer s.:
Hours per week, examination:	summer s.:2/2, C [HT]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	cancelled
Language:	English
Teaching methods:	full-time
Teaching methods:	full-time
Level:
For type:

Guarantor:	doc. MUDr. Jaromír Kotyza, CSc.
Is co-requisite for:	EA0903010

Opinion survey results Examination dates Schedule Noticeboard

Annotation -

Last update: MUDr. Vlastimil Kulda, Ph.D. (15.11.2017)

Proteins, structure and function. Enzymes: classification, function, regulation. Biochemical thermodynamics, energy metabolism, respiratory chain, oxidative phosphorylation. Glycolysis and citric acid cycle. Metabolism of saccharides and lipids.

Literature - Czech

Last update: MUDr. Vlastimil Kulda, Ph.D. (16.11.2017)

Obligatory literature:

Kotyza J. et al.: Clinical Chemistry and Enzymology - Laboratory Manual. Charles University, Faculty of Medicine in Pilsen, Plzen 1998.

Ferrier D.R. et al.: Lippincott Illustrated Reviews: Biochemistry (7th Edition). Wolters Kluwer, Philadelphia 2017.

Recommended literature:

Rodwell V.W. et al.: Harpers Illustrated Biochemistry (30th Edition). McGraw-Hill 2015.

Requirements to the exam

Last update: Vendulka Votípková (25.05.2018)

Semester credits will be granted based on:
1. A complete attendance at laboratory classes. The missing laboratories must be substituted.
2. Presentation of laboratory protocols.
3. Passing both credit tests. The number of attempts is limited to three per test!

Syllabus -

Last update: MUDr. Vlastimil Kulda, Ph.D. (16.11.2017)

Laboratories and Seminars:

Enyzymology. Physiology of renal functions. Glomerular filtration. Examination of urine (physical, chemical, microscopic).

Lectures:

Introduction to medical biochemistry. Metabolic reactions. Cellular compartments and their biochemical functions. Catalysis, biocatalysis, properties of enzymes.

Structure and function of proteins - recapitulation. Mechanisms of enzymic catalysis. Classification of enzymes. Kinetics of enzymic reactions. Allosteric enzymes. Inhibitors of enzymic reactions.

Thermodynamics of biochemical processes. High-energy carriers. Redox reactions in biochemistry. An overwiev of the respiratory chain.

Coenzymes of the respiratory chain. Oxidative phosphorylation.

The basic strategy in the extraction of energy from foodstuffs. Digestion of hydrocarbons. Glycolysis under aerobic and anaerobic conditions. Oxidative decarboxylation of pyruvate.

Gluconeogenesis. The citric acid cycle and its regulation, energy yield, anaplerotic reactions.

Pentose phosphate pathway. Glycogen metabolism and its regulation. UDP-derivatives of saccharides.

Metabolism of galactose and fructose. Glycoproteins and proteoglycans. Digestion and absorption of lipids.

Lipolysis in adipocytes. Degradation and biosynthesis of fatty acids. Ketogenesis.

Lipoproteins. Phospholipids and glycolipids. The structure of biomembranes. Metabolism of unsaturated fatty acids. Synthesis of eicosanoids.

Cholesterol and its biosynthesis. Biosynthesis of bile acids. Calcitriol. Formation of steroid hormones. Integration of hydrocarbon and lipid metabolism.