Microbiology I. - DA1104339

• Title: Microbiology I.
• Guaranteed by: Department of Medical Microbiology (13-323)
• Faculty: Second Faculty of Medicine
• Actual: from 2023
• Semester: summer
• Points: 5
• E-Credits: 5
• Examination process: summer s.:
• Hours per week, examination: summer s.:28/42, C [HS]
• Extent per academic year: 14 [weeks]
• Capacity: unlimited / unlimited (unknown)
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Guarantor: prof. MUDr. Pavel Dřevínek, Ph.D.
• Classification: Medicine > Basic Sciences
• Co-requisite : DA1103040
• Pre-requisite : DA1101001, DA1101338, DA1102338
• Is pre-requisite for: DA1105038, DA1105439

Annotation

Last update: Jan Kovář (01.07.2022)

Basic principles of general microbiology, patogenetic mechanisms, special bacteriology, mycology, virology and parasitology, principles of antimicrobial therapy, antiinfectious immunity and specific and nonspecific immunomodulation, clinical microbiology with selection of laboratory techniques, interpretation, differential diagnostics.

Course completion requirements

English

Last update: Romana Laušerová (09.02.2024)

Mandatory participation in practical exercises with one permitted absence. It is possible to replace the practice with another circle twice. Other refunds will not be allowed. If the student does not have enough attendance, the student will be tested by the teacher.

"The passing of test (the test includes 10 points in total; 6 points are the threshold to pass) is a condition for getting the credit. Individual test has to be completed by every student before the start of the lecture (13th week)".

And successfully passing practical exam at the last week of the practicals.

Czech

Last update: Jan Kovář (01.07.2022)

Mandatory participation in practical exercises with one permitted absence. It is possible to replace the practice with another circle twice. Other refunds will not be allowed. If the student does not have enough attendance, the student will be tested by the teacher.

"The passing of test (the test includes 10 points in total; 6 points are the threshold to pass) is a condition for getting the credit. Individual test has to be completed by every student before the start of the lecture (13th week)".

Literature

English

Last update: Jan Kovář (01.07.2022)

Murray, P. Medical microbiology : Mosby, 2009 (B), (avaible as ebook)
+Mims, C. A.: Medical microbiology : Mosby, 2013

Czech

Last update: Jan Kovář (01.07.2022)

Bednář M., Fraňková V., Schindker J. akol., Lékařská mikrobiologie 1992, Marvil Praha

Syllabus

Last update: Jan Kovář (01.07.2022)

Syllabus - international students practical course

Medical Microbiology - 2nd year - summer semester

1) Laboratory safety rules and equipment. Processing of clinical samples in microbiological analysis.

Medical students will be familiarized with safety rules and with organization of education process at the Department of Medical Microbiology. Information including desinfection and sterilization will be presented and various microscopic will be demonstrated.

2) Collection of clinical samples and transport.

Demonstration of relevant collection procedures of significant clinical samples.

3) Direct detection, part 1 (microscopy).

Morphology of bacterial cell; microscopy preparation and fixation; various staining procedures.

4) Direct detection, part 2 (cultivation, phenotypic identification, serotyping).

The goal of the practical is cultivation of microorganisms using general, enriched and diagnostic culture media. Students are familiarized with fundamental terms as culture media and cultivation conditions. Students practise inoculation on culture media. The practical includes working with isolates, their identification with phenotypic method and reverse agglutination.

5) Susceptibility to antibiotics.

Students determine antibiotic susceptibility of various clinical bacterial isolates by using disc diffusion method. Minimum inhibitory concentration (MIC) using broth microdilution method and E-test will be demonstrated.

6) Indirect serological methods.

The most significant indirect (serological) methods (agglutination, precipitation, Enzyme-Linked ImmunoSorbent Assay - ELISA) are introduced. Serological results of the methods are presented and interpreted using knowledge of basic immunological mechanisms.

7) Molecular biology methods.

Overview of molecular biology methods applicable in medical microbiology laboratory.

8) Staphylococci.

Microbiological diagnosis of staphylococcal infection. Phenotypic identification using screening tests, ie. free and bound coagulase, catalase test.

9) Streptococci.

Differentiation of streptococci using their colony morphology, hemolytic properties and various tests as optochine test, PYR test, CAMP test.

10) Bordetella, Haemophilus, Neisseria.

Demonstration of supplemented enriched media which should be used for cultivation of the fastidious organisms. Instruction for deep nasopharyngeal swab to collect clinical material for detection of Bordetella pertussis or B. parapertussis.

11) Enterobacteriaceae.

Microbiological diagnosis of clinically important strains. Practical approach of reverse agglutination to confirm relevant serotypes.

12) Pseudomonas and Nonfermenters.

Microbiological diagnosis of significant clinical species. Detection of various pigment production and antibiotic resistance of clinical isolates.

13) Listeriae and Corynebacteria.

Cultivation of saprophytic and pathogenic corynebacteria using diagnostic culture media. Detection of strain toxigenity. Temperature dependent cultivation of Listeria monocytogenes.

14) Credits.