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IIA - Immunopathology - CTBSIP13

Title:	IIA - Imunopatologie
Guaranteed by:	Department of Immunology and Clinical Biochemistry 3FM CU (12-IMUN)
Faculty:	Third Faculty of Medicine
Actual:	from 2016
Semester:	winter
Points:	1
E-Credits:	1
Examination process:	winter s.:
Hours per week, examination:	winter s.:12/4, other [HS]
Extent per academic year:	16 [hours]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	not taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Additional information:	http://www.lf3.cuni.cz/studium/materialy/imunologie/

Guarantor:	doc. MUDr. Petr Kučera, Ph.D.
Classification:	Medicine > Basic Sciences
Attributes:	Modul IIA

Examination dates Schedule

Annotation -

Last update: KUCERA2 (30.05.2008)

The Course of Module IIA covering more advanced mechanisms of immunity and dominantly abnormal functions of immune system

Aim of the course -

Last update: KUCERA2 (30.05.2008)

The goal of the Course is to understand to pathofysiological characteristics of abnormal immune reactions, to help to explain mechanisms of immune mediated diseases.

Literature -

Last update: KUCERA2 (30.05.2008)

Roitt I, Delves PJ: Essential Immunology (Blackwell Scientific Publications 2001, 10th ed)

Kuby J: Immunology, New York : W.H. Freeman, 1992

Teaching methods -

Last update: KUCERA2 (30.05.2008)

Lectures and two practicum are used, with the demonstration of immunology laboratory tests. Students are asked to evaluate results of those tests, case studies are used to demonstrate usefulness of immunology diagnostics.

Requirements to the exam -

Last update: KUCERA2 (15.08.2008)

1. Written test for the Credit (multiple choise), the position in 80 percentile is required

2.The Exam from Immunopathology is a part of the final exam Module IIA - written multiple choice test based on topics in the course lectures and practicum.

Syllabus -

Last update: KUCERA2 (15.08.2008)

Lectures:

1. Antibody immune response and regulation. T-independent, T-dependent antigens. Immunology basis of vaccination

2. Immune function of T cells - Th1, Th2, Th17, regulatory T cells. Cytotoxic reactions, NK, NKT cells

3. Mucosal and skin immune reaction. Inflammation and immune reaction

4. Break of the immune tolerance, autoimmunity-mechanisms and risk factors

5. Antiinfection immunity. Prions and the immune system. Primary immunodeficiency - molecular basis

6. Immunopathology reactions of hypersensitivity, Coombs and Gell Type I - IV

Practicum I:

Immune proteins analysis - immunoglobulins, specific antibodies (against infectious and other antigens), acute phase proteins, paraproteins

Methods for detection of autoantibodies - indirect immunofluorescence, ELISA, Western Blott (WB)

Complement tests, tests of the complement function (including complement inhibitors)

Theoretical principles of: immunodifusion, precipitation, agglutination, immunoelectrophoresis / immunofixation, radio/enzymoimmunoanalysis, turbidimetry and nephelometry.

Practical demonstration: typical patterns of autoantibodies detection by indirect immunofluorescence and westernblott

Based on case studies

Practicum II:

Methods for analysis of cell mediated immunity

Flow cytometry - principle of the method. Phenotypization of the cells - application in different areas. Analysis of the activation state of leucocytes, thrombocytes and other cells.

Functional tests of lymphocytes - cytotoxicity, proliferation, cytokine secretion

Functional tests of phagocyting cells - ingestion, metabolic burst of Neu

Practical demonstration: analysis of membrane markers of peripheral blood lymphocytes

Based on case studies

Entry requirements -

Last update: KUCERA2 (30.05.2008)

The basis of knowledge of cell and molecular immunology