|
|
|
||
|
The aim of the course is to elucidate the principles of the cell differentiation in the ontogenesis of the animal organisms. The main topics are genome stability and variability during embryogenesis, gene activity from the genetic and epigenetic point of view, processing and regulation of gene expression, inter-cellular relationships, propagation of inter-cellular signals, role of the extracellular matrix. Inductive interaction, morphogenetic field and positional information, hierarchy of genetic activities in morphogenesis, in the formation of the spatial orientation of animal body.
Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (17.02.2021)
|
|
||
|
The course is based on the recent reviews, original articles and worldwide textbooks (i.e. Gilbert et al., Developmental biology). Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (30.05.2019)
|
|
||
|
Subject organization: In summer semester 2024/2025 lectures from Cell differentiation in ontogenesis start on Monday 17th February 2025 from 10:40 to 12:10 in the Lecture Hall Fotochemie (B3), Vinicna 7. ground floor left. Lectures are taught in hybrid format with simultaneous personal presentation and real time online broadcasting via Google Meet and video recording. I would like to invite you to enroll to Google Classroom course Cell Differentiation in Ontogenesis where you find my presentations, recordings from online lectures or interesting review articles. Password: i5inh6z. After enrolment you will also have access to Google Meet online link and Google Calendar (Cell Differentiation in Ontogenesis) with scheduled lectures. If you have any questions, don´t hesitate and write me an e-mail (vkrylov@natur.cuni.cz)
The exam is oral (personal meeting or online via Google Meet) Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (18.01.2025)
|
|
||
|
1. Life cycle, cell differentiation
Genome stability and variability during embryonic development. Chromatin diminution, multiplication, transposition, gene rearrangement during cell differentiation. Somatic cell nucleus transfer (SCNT).
2. Differential gene activity and affinity of cells during embryonic development
Regulation of gene expression and its mechanisms. Genetic and epigenetic factors.
3. Inter-cellular relationships in the embryonic development
Endo, auto, para, juxtracrine signaling. Inter-cellular signals, receptors, and their utilization. Neural induction as an example.
4. Gametogenesis
Principles of gonocyte differentiation. Structure and molecular basis of gametes and their importance for the development. Maternal influence on the embryonic development mediated by oocyte. Mosaic and conditional development. Gradient of morphogens.
5. Body axis symmetry I
Morphogenesis and spatial orientation in amphibians. Inductive interaction as a principle of morphogenetic regulation. Mesodermal, neural, and other inductions. Experimental evidence of inductions and their molecular basis. Mechanisms of anterior-posterior (A-P) and dorso-ventral (D-V) axis symmetry in Xenopus model.
6. Body axis symmetry II
Body axis symmetry in fish, birds, and mammals. EVO-DEVO comparison of the A-P and D-V axis origin.
7. Body axis symmetry III
A-P and D-V axis symmetry in Drosophila melanogaster. Left-right symmetry in animals.
8. Regeneration and aging
Embryonic and tissue specific stem cells, their features and utilization. Types of regeneration, ability of variable tissues and organisms to regenerate.
The course is taught with the support of the project reg. number CZ.02.2.69/0.0/0.0/16_015/0002362 Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (17.02.2022)
|
|
||
|
Zvládnout výuku v anglickém jazyce. Zkouška je pro česky mluvící studenty vedena v češtině. Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (17.02.2021)
|