Subjects

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Modern trends in liquid chromatography - GDBM07

Title:	Moderní trendy v kapalinové chromatografii
Guaranteed by:	Department of Analytical Chemistry (16-16140)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2019
Semester:	both
Points:	0
E-Credits:	0
Examination process:
Hours per week, examination:	0/0, Ex [HT]
Capacity:	winter:unknown / unknown (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Teaching methods:	full-time
Level:
Note:	course is intended for doctoral students only enabled for web enrollment you can enroll for the course in winter and in summer semester

Guarantor:	prof. PharmDr. Lucie Nováková, Ph.D.
Interchangeability :	GDZCH07
Is interchangeable with:	GDZCH07

Examination dates WS schedule SS schedule

Annotation -

Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (01.03.2018)

The aim of the subject is to extend the knowledge in the field of liquid chromatography method with an emphasis on modern trends in this field. These include ultra-high performance liquid chromatography, core-shell particles, monolithic sorbents, and high temperature liquid chromatography, new stationary phase sorbents, and detection-based mass spectrometry detection. From a practical point of view, the course will cover LC methods development and troubleshooting. An important modern trend includes supercritical fluid chromatography.

Literature - Czech

Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (01.03.2018)

Povinná:

Nováková, Lucie, Douša, Michal. Moderní HPLC separace v teorii a praxi. Praha: Europrint, 2013, 299 s. ISBN 978-80-260-4243-3.

Nováková, Lucie, Douša, Michal. Moderní HPLC separace v teorii a praxi. Praha: Europrint, 2013, 235 s. ISBN 978-80-260-4244-0.

Doporučená:

Guillarme, Davy Veuthey, Jean-Luc (eds.). UHPLC in life sciences. Cambridge: The Royal Society of Chemistry, 2012, 447 s. ISBN 978-1-84973-388-5.

. . In Journal of Chromatography A. (): -.

. . In Journal of Separation Science. (): -.

Web of Knowledge [online]. Dostupné z: http://webofknowledge.com

Requirements to the exam -

Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (01.03.2018)

(1) Modern Trends in Liquid Chromatography

van Deemter theory, ultra-high performance liquid chromatography (UHPLC), surface porous particles, monolithic columns, high temperature liquid chromatography, modernization of instrumentation in the field of separation methods

(2) New types of stationary phases in HPLC

Stationary phase based on silica gel, organic polymers, zirconia and porous graphite carbon, hybrid stationary phase, chiral separation

(3) Development of HPLC method

Selection of stationary and mobile phase conditions, analysis of basic substances

(4) Troubleshooting in HPLC

Problem Solving in Liquid Chromatography - Pressure, Baseline, Retention Time, Peak Counts

(5) Supercritical Fluid Chromatography (SFC)

Method principle, stationary and mobile phase, detection, application

(6) Liquid and supercritical fluid chromatography combined with mass detection

The need for chromatographic separation, aspects of LC, SFC and MS parameters for the combination of these techniques