Biology I - FD10009

• Title: Biology I
• Guaranteed by: Department of Medical Biology and Genetics (15-120)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2020
• Semester: winter
• Points: 0
• E-Credits: 4
• Examination process: winter s.:
• Hours per week, examination: winter s.:15/30, C [HS]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: not taught
• Language: English
• Teaching methods: full-time
• Teaching methods: full-time
• Guarantor: prof. PharmDr. Emil Rudolf, Ph.D.
• Incompatibility : FZ10009
• Interchangeability : FA0201045, FZ10009
• Is co-requisite for: FD10010
• Is incompatible with: FZ10009
• Is interchangeable with: FZ10009
• In complex pre-requisite: FDP024

Annotation

Last update: Marcela Laláková (25.09.2018)

Definition of life, organisation of living systems. Molecules in cells, structure and function of proteins and nucleic acids. Cell theory, prokaryotic and eukaryotic cells. The cell nucleus, organisation of DNA, chromosomes. DNA replication and repair. Transcription and translation of genetic information, RNA processing, posttranslational modification. The cellular membranes, structure and function. Transport across biomembranes, cell membrane fusion. The endoplasmic reticulum, the Golgi apparatus. The mitochondrion, production of ATP. The cytoskeleton. Cell growth and cell division. Regulation of cellular functions. Cell death, necrosis, apoptosis.

Requirements to the exam

Last update: Marcela Laláková (25.09.2018)

I.  General

· Attendance at practical classes is mandatory.

· Practicals begin at 3,00 p.m. and student is required to be in his seat 5 minutes before the beginning of the class.

· Each student is allowed one absence from one practical class. Medical excuse or any other valid official reason is required. (delivered within max. 48 h after the missed class).

· A clean, dry pair of indoor shoes (not boots) is required. If a student does not have clean shoes, he can purchase disposable shoe covers. Backpacks and coats must be stored away from the working area.

· White lab coats must be worn for all practical classes.

· No eating, drinking or smoking in the laboratory at any time.

· In case of injury, report it immediately to the instructor.

· The clean-up procedure for each practical class will be explained at the beginning of the course.

· Deadline for sending via email a full-text medical article is November 30^th 2018. The topic of the article may be used in the final semestral presentation.

 

II. Credit

 

The credit for Medical Biology practical classes in winter semester consists of:

· Completed Labbook. Labbook presented during credit session must have an appropriate external form; i.e. be clean, intact as it has status of official document. In case of any workbook damage or loss, the student has to acquire a new one from the department against financial compensation.

· Final test with minimal scoring grade of 75 % (there is one final test and in case of its failure, there are two more retakes).

· Fulltext of selected biomedical article (winter term)

· Presentation of selected topic (winter term)

 

Cheating during any form of biology exam (credit test and oral examination) including preparatory period is not tolerated! Use of any electronic devices (cell phones, tablets, notebooks, phablets etc.), unauthorized materials or communication with other students in these instances is strictly prohibited! 

 

Appointments for respective credits are scheduled to winter examination period (January 21^th 2019 – February 15^th 2019) and summer examination period (June 3^th 2019 – June 30^th 2019) and during September (2^nd – 20^th 2018), each week on Thursdays from 2 to 3 pm only. 

Each student has to sit credit at least once in winter examination period. Generally, in case of failure in credit (i.e. final test or incomplete manual), the next possible chance is after one week!!!!

 

The students who seek the exemption from practical classes in medical biology and genetics have to submit their request before the start of practical classes. Their request must be accompanied by a detailed syllabus of biology course they had completed which will be evaluated by the head of the department of medical biology and genetics.

Syllabus

Last update: Marcela Laláková (25.09.2018)

Lecturers

• RNDr. Věra Králová, Ph.D.

Lectures  

1. Organisation of living systems – definition of life, cell theory. Models in biomedicine. Prokaryotic and eukaryotic cells.
   DNA synthesis.
   Expression of genetic information 1  – transcription,post-transcriptional modifications of RNA
2. Expression of genetic information 2 – transcription, post-transcriptional modifications of RNA, translation, post-translational modifications of proteins.
   Cell membranes – structure and function of biological membranes, membrane transport, membraneous organelles of eukaryotic cells.
3. Mitochondrion – structure and function, ATP production.
   Cytoskeleton – microtubules, microfilaments, intermediate filaments, cellular movements, transport of vesicles.
4. Regulation of cellular functions – principles, signals, receptors, signaling pathways, regulation of gene expression.
   Cell cycle - course and regulation.
   Cell death.
5. Introduction to genetics – genes, genomes, variability of individuals. Mutations, polymorphisms, reparatory mechanisms.
   Human genome – organisation of genome, chromosomes, structure of chromatin and its changes.
   Meiosis.

 

Practical courses & seminars

Lecturers

• Prof. PharmDr. Emil Rudolf, Ph.D.
• RNDr. Veronika Skarková, Ph.D.

Practical course

1.         Microscopic techniques I.

1.1       General terms and conditions of the biology course

1.2       Size estimation

1.3       Permanent mount of cells cultivated in vitro

 

2.         Microscopic techniques II.

2.1       Wet mount of buccal cells

  

3.         Cellular membranes – osmotic phenomena

3.1       Osmotic changes in erythrocytes after the treatment with anisotropic solutions

3.2       Plasmolysis in plant cell (Elodea densa)

  

4.         Cell motility

4.2       Cyclosis in plant cells (Elodea densa)

4.3       Amoeboid movement

4.4       Flagellar movement

  

5.         Cell cultures in biomedicine (seminar)

5.1       Cell and tissue culture techniques

 

6.         Information sources and their relevance in biomedicine (seminar)

6.1       Scientific information in biomedicine – the structure of scientific article

6.2       In vitro tests used for cytotoxicity assays – dental applications

  

7.         Cell death – scientific methodology

7.1       Permanent mount of tumor cells treated with cytostatic drug

7.2.      Vital staining

  

8.         Cell cycle and cell division

8.1       Mitosis in permanent mounts of plant cells

8.2       Mitosis in animal cell cultures

8.3       Mitotic index and particular phases of mitosis

  

9.         Advances in biosciences (seminar)

9.1       Student presentations (biodentistry oriented)

  

10.       CREDIT

10.1     Final test

 

 

 

Literature

Last update: Marcela Laláková (25.09.2018)

Compulsory literature

1. Manuals and other study materials - MOODLE
2. Alberts, B. et al.         Essential Cell Biology, 4th edition, Garland Science, 2013