Advanced Techniques in Microbiology - FV139

• Title: Advanced Techniques in Microbiology
• Guaranteed by: Department of Clinical Microbiology (15-560)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2023
• Semester: summer
• Points: 0
• E-Credits: 1
• Examination process: summer s.:
• Hours per week, examination: summer s.:9/6, C [HS]
• Capacity: 9
• Min. number of students: 5
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: doc. MUDr. Miroslav Fajfr, Ph.D.
• Pre-requisite : {Malá skupina pro FV039}
• Incompatibility : FA0105111, FG50026
• Interchangeability : FG50026

Annotation

Advanced molecular techniques are now routinely used in the microbiological laboratory. Student of this course will thus learn common molecular methods, such as PCR and its modifications, MALDI-TOF, next generation sequencing (NGS) and others utilized in microbiology. In addition, the students will acquire knowledge about specific incorporation of these methods in the clinical diagnostic procedures. The course is primarily aimed at students interested in scientific work. During the first practical, the students will perform an isolation of nucleic acids, PCR setup and subsequent detection using gel electrophoresis. The second practical will aim at performing an identification of bacterial cultures using MALDI-TOF. For practical exercises, it is necessary to divide the students into groups of 4 students. The reason is the practical implementation of the PCR reaction and work with MALDI-TOF MS, which cannot be carried out in larger numbers (practical involvement of students would not be possible). For that reason, the inclusion of practical exercises in the schedule is only indicative, and the actual implementation of practical exercises will be agreed upon by the teacher individually with the students according to their schedule at the last seminar.

Last update: Dyntarová Eva (31.01.2025)

Literature

Recommended literature:

1. Goering R, et al. Mims' Medical Microbiology and Immunology, 6th Ed., Elsevier, 2018.
2. Greenwood D. Medical Microbiology, 18^th Ed., Elsevier, 2012.

Last update: Dyntarová Eva (31.01.2025)

Requirements to the exam

Credit:

1.  full attendance required (absences are not allowed)

Last update: Dyntarová Eva (31.01.2025)

Syllabus

Seminars

 1.    Polymerase chain reaction (PCR) and its modifications (3hrs) – theory of PCR, both classical and advanced modifications

2.    Sequencing (3hrs) - theory of sequencing reactions, Sanger sequencing, NGS and their use in the microbiological diagnosis and research

3.    MALDI-TOF and its applications (3hrs) - principles and methodology of mass spectrometry

 

Practical classes

 1.    PCR - isolation of nucleic acids, setting up a PCR reaction, detection of products using gel electrophoresis (3-4hrs)

2.    MALDI-TOF - practically performing an identification of cultures (3hrs)

 

Lecturers:

• MUDr. Miroslav Fajfr, Ph.D.
• Mgr. Radka Bolehovská, Ph.D,
• Mgr. Rudolf Kukla, Ph.D.

 

Last update: Dyntarová Eva (31.01.2025)