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The Course of Module IIA covering more advanced mechanisms of immunity and dominantly abnormal functions of immune system
Last update: KUCERA2 (30.05.2008)
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The goal of the Course is to understand to pathofysiological characteristics of abnormal immune reactions, to help to explain mechanisms of immune mediated diseases. Last update: KUCERA2 (30.05.2008)
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Roitt I, Delves PJ: Essential Immunology (Blackwell Scientific Publications 2001, 10th ed) Kuby J: Immunology, New York : W.H. Freeman, 1992 Last update: KUCERA2 (30.05.2008)
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Lectures and two practicum are used, with the demonstration of immunology laboratory tests. Students are asked to evaluate results of those tests, case studies are used to demonstrate usefulness of immunology diagnostics. Last update: KUCERA2 (30.05.2008)
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1. Written test for the Credit (multiple choise), the position in 80 percentile is required 2.The Exam from Immunopathology is a part of the final exam Module IIA - written multiple choice test based on topics in the course lectures and practicum. Last update: KUCERA2 (15.08.2008)
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Lectures: 1. Antibody immune response and regulation. T-independent, T-dependent antigens. Immunology basis of vaccination 2. Immune function of T cells - Th1, Th2, Th17, regulatory T cells. Cytotoxic reactions, NK, NKT cells 3. Mucosal and skin immune reaction. Inflammation and immune reaction 4. Break of the immune tolerance, autoimmunity-mechanisms and risk factors 5. Antiinfection immunity. Prions and the immune system. Primary immunodeficiency - molecular basis 6. Immunopathology reactions of hypersensitivity, Coombs and Gell Type I - IV
Practicum I: Immune proteins analysis - immunoglobulins, specific antibodies (against infectious and other antigens), acute phase proteins, paraproteins Methods for detection of autoantibodies - indirect immunofluorescence, ELISA, Western Blott (WB) Complement tests, tests of the complement function (including complement inhibitors) Theoretical principles of: immunodifusion, precipitation, agglutination, immunoelectrophoresis / immunofixation, radio/enzymoimmunoanalysis, turbidimetry and nephelometry. Practical demonstration: typical patterns of autoantibodies detection by indirect immunofluorescence and westernblott Based on case studies
Practicum II: Methods for analysis of cell mediated immunity Flow cytometry - principle of the method. Phenotypization of the cells - application in different areas. Analysis of the activation state of leucocytes, thrombocytes and other cells. Functional tests of lymphocytes - cytotoxicity, proliferation, cytokine secretion Functional tests of phagocyting cells - ingestion, metabolic burst of Neu Practical demonstration: analysis of membrane markers of peripheral blood lymphocytes Based on case studies Last update: KUCERA2 (15.08.2008)
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The basis of knowledge of cell and molecular immunology Last update: KUCERA2 (30.05.2008)
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