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Course, academic year 2012/2013
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Laboratory Course on Separation Methods - MC230C16
Title: Praktikum ze separačních metod
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2011 to 2012
Semester: summer
E-Credits: 4
Examination process: summer s.:
Hours per week, examination: summer s.:0/2, C [DS]
Extent per academic year: 4 [days]
Capacity: 20
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: specialized
Note: priority enrollment if the course is part of the study plan
Guarantor: prof. RNDr. Zuzana Bosáková, CSc.
Teacher(s): prof. RNDr. Zuzana Bosáková, CSc.
doc. RNDr. Tomáš Křížek, Ph.D.
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download HPLC Chirální separace2012.pdf prof. RNDr. Zuzana Bosáková, CSc.
Annotation -
Last update: prof. RNDr. Zuzana Bosáková, CSc. (29.03.2016)
The laboratory course of separation methods trains experimental skills in advanced instrumental analytical techniques.
Literature - Czech
Last update: prof. RNDr. Zuzana Bosáková, CSc. (24.09.2014)

Analytické separační metody, K. Štulík a kol., Karolinum, Praha, 2005.

Encyclopedia of Separation Science. M. Crooke, C. F. Poole (Eds.) Academic Press 2000

Chromatography today, C.F. Poole, S.K. Poole, Elsevier, Amsterdam, 1991.

Requirements to the exam - Czech
Last update: doc. RNDr. Tomáš Křížek, Ph.D. (05.09.2023)

Účast v praktiku, vypracování příslušných protokolů a přezkoušení u jednotlivých úloh.

Syllabus -
Last update: doc. RNDr. Tomáš Křížek, Ph.D. (05.09.2023)

1) Optimization of chiral separation will be demonstrated on a drug from the group of b-blockers. The enantioselective separation system will be composed of a chiral stationary phase based on macrocyclic antibiotics and achiral mobile phase.

2) The separation and quantification capabilities of the HPLC-MS/MS will be demonstrated on the analysis of selected organic pollutants.

3) This practical course which demonstrates features of fast gas chromatography (fast-GC) utilizing capillary columns of very small diameters should make students familiar with this modern kind of GC and show them an example of fast analysis of selected group of pollutants. This exercise facilitates completion of knowledge and experience additionally to the classical GC methods learned in previous practical courses.

4) Analysis of sodium and potassium ions in urine by capillary electrophoresis.

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