SubjectsSubjects(version: 945)
Course, academic year 2023/2024
   Login via CAS
Scattering Methods in Optical Spectroscopy - NOOE012
Title: Rozptylové metody v optické spektroskopii
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2023
Semester: summer
E-Credits: 3
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: prof. RNDr. Marek Procházka, Ph.D.
prof. RNDr. Vladimír Baumruk, DrSc.
RNDr. Václav Profant, Ph.D.
Classification: Physics > Biophysics and Chemical Physics, Optics and Optoelectronics
Annotation -
Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)
Techniques of quasielastic, Brillouin and Raman scattering and their application to inorganic, organic and biological materials. Resonance and surface-enhanced Raman scattering. Non-linear techniques in Raman spectroscopy. Time-resolved Raman scattering.
Aim of the course -
Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)

Students will be acquainted with the techniques of optical spectroscopy based on light scattering and their applications in physics.

Course completion requirements
Last update: prof. RNDr. Vladimír Baumruk, DrSc. (30.10.2019)

Oral Exam, 3 questions

Literature -
Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)

Harris D. C., Bertolucci M. D.: Symmetry and Spectroscopy (selected chapters), Oxford University Press, New York 1978.

Demtröder W: Laser spectroscopy (chapters 4 and 9), Springer, Berlin 1981.

Methods of Experimental Physics vol. 20: Biophysics (Ehrenstein G, and Lecar H., Eds.) (chapters 3 a 7), Academic Press, New York 1982.

Twardowski J., Anzenbacher P.: Raman and IR Spectroscopy in Biology and Biochemistry, Ellis Horwood, Chichester 1994.

Carey P. R.: Biochemical Applications of Raman and Resonance Raman Spectroscopies, Academic Press, New York 1982.

Creery R. L.: Raman Spectroscopy for Chemical Analysis, Wiley-Interscience, New York 2000.

Handbook of Vibrational Spectroscopy vol. 1-5 (Chalmers J.M., and Griffiths, Eds.), John Wiley and Sons, Chichester 2002.

Teaching methods -
Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)


Syllabus -
Last update: T_FUUK (14.05.2004)
1. Scattering of light in nature.
Basic classification of scattering processes - elastic and inelastic light scattering. Inealstic light scattering and optical spectroscopy.

2. Quasi-Elastic (dynamic) light scattering (QELS).
Correlations in time and space: relationships to frequency spectra. Dynamic theory of light scattering. Principles of experimental detection, homodyne and heterodyne methods. Aplications in biophysics, translational and rotational diffusion, polydispersity, intramolecular motion.

3. Brillouin scattering.
Basic principles. Experimental techniques, Fabry-Perot interferometers. Aplications of Brillouin scattering to liquids, polymers and solids.

4. Spontaneous Raman scattering.
Theoretical background, classical framework, quantum mechanical framework. Polarizability, scattering tensor, depolarization ratio. Non-resonance Raman scattering - basic properties, selection rules. Raman scattering as a technique of vibrational spectroscopy, comparison to infrared absorption spectroscopy. Resonance Raman scattering. Surface enhanced Raman scattering (SERS). Raman optical activity (ROA).

5. Modern Raman instruments and techniques.
Components of Raman spectrometers- excitation lasers, spectrographs, photomultipliers, multichannel detectors. Polarized measurements in Raman spectroscopy. Sample handling, gaseous, liquid and solid samples, singl ecrystals, powders, thin films. Signal-to-noise ratio and its improvement. Difference techniques.Time-resolved Raman spectroscopy. Micro-Raman spectroscopy and Raman imaging.

6. Interpretation of Raman spectra.
Molecular symmetry and selection rules in vibrational spectroscopy. Interpretation of vibrational spectra, band assignment. Structural sensitivity, conformational markers. Biophysical applications of Raman spectroscopy.

7. Nelinear techniques in Raman spectroscopy.
Hyper-Raman effect. Stimulated Raman scattering and its applications. Four-photon techniques - coherent anti-Stokes Raman scattering (CARS), inverse Raman and stimulated Raman gain spectroscopy. Time-resolved CARS.

Charles University | Information system of Charles University |