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Course, academic year 2019/2020
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X-ray Structure Analysis and Electron Microscopy - NFPL025
Title in English: Rentgenová strukturní analýza a elektronová mikroskopie
Guaranteed by: Department of Condensed Matter Physics (32-KFKL)
Faculty: Faculty of Mathematics and Physics
Actual: from 2016
Semester: winter
E-Credits: 3
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Guarantor: prof. RNDr. Radomír Kužel, CSc.
doc. RNDr. Bohumil Smola, CSc.
Classification: Physics > Solid State Physics
Incompatibility : NFPL012
Annotation -
Last update: VALVODA (03.05.2004)
Crystallography of solids and biological objects. Study of structure of crystals, molecules and biological objects by X-ray diffraction and electron microscopy.
Literature - Czech
Last update: VALVODA (03.05.2004)

1) V. Valvoda, M. Polcarová, P. Lukáč: Základy strukturní analýzy, Karolinum, Praha 1992.

2) V. Prosser a kol.: Experimentální metody biofyziky, Academia, Praha 1989.

3) G. Giacovazzo et al.: Fundamentals of Crystallography 2nd Edition, IUCr, Oxford Science Publications, Oxford 2002.

4) L. V. Azároff: Elements of X-ray Crystallography, McGraw-Hill Book Company, New York 1968.

5) B. Smola: Transmisní elektronová mikroskopie ve fyzice pevných látek (skripta), SPN, Praha 1983.

Syllabus -
Last update: T_KFES (21.05.2004)
Crystallographic symmetry operations, point groups, crystallographic systems, lattices, space groups, symmetry of cylindrical and helical molecules, symmetry of spherical viruses.

Basic diffraction conditions
Reciprocal lattice, diffraction conditions, Bragg´s law, geometry of basic diffraction methods, Ewald´s construction.

Kinematical theory
Atomic scattering factor, structure factor, interference function, intensity of X-ray reflections in maximum and integrated intensity, peak width.

Experimental methods of structure analysis
Stereographic projection and crystal orientation by the Laue method, the method of rotating crystal, Weissenberg method, precession method, X-ray diffractometers, powder method of Debye and Scherrer

Structure analysis
Fourier transformation of electron density, "phase problem", methods of crystal structure solution, structure refinement

Application of structure analysis in materials research
Determination of lattice parameters, identification and quantitative phase analysis, texture, macroscopic and microscopic stresses, size of crystallites

Biological materials
Structure determination of fiber molecules, globular proteins, spherical viruses, study of interatomic bonds, reconstruction of structure from electron micrographs, time lenses.

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