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Course, academic year 2018/2019
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Modern Measuring and Data Analysis Methods in Time Resolved Fluorescence Spectroscopy - NBCM313
Title in English: Moderní metody měření a analýzy dat v časově rozlišené fluorescenční spektroskopii
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2017
Semester: winter
E-Credits: 3
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: not taught
Language: Czech
Teaching methods: full-time
Note: enabled for web enrollment
Guarantor: doc. RNDr. Jaroslav Večeř, CSc.
prof. RNDr. Petr Heřman, CSc.
Classification: Physics > Biophysics and Chemical Physics
Annotation -
Last update: VECER/MFF.CUNI.CZ (16.05.2008)
Advanced techniques of the time resolved fluorescence spectroscopy - data acquisition and analysis in time domain
Aim of the course -
Last update: VECER/MFF.CUNI.CZ (16.05.2008)

To learn advanced techniques of the time resolved fluorescence spectroscopy - data acquisition and analysis in time domain

Literature -
Last update: T_FUUK (28.04.2008)

Lakowicz J. R. Principles of fluorescence spectroscopy, Second edition. New York: Kluwer Academic/Plenum Publishers, 1999.

Večeř J., Kowalczyk A. A. and Dale R. E., Improved recursive convolution integral for the analysis of fluorescence decay data: Local approximation of the apparatus response function by a general polynomial. Rev.Sci.Instrum. 64 (1993) 3403-3412

Večeř J., Kowalczyk A. A., Davenport L. and Dale R. E., Reconvolution analysis in time-resolved fluorescence experiments - an alternative approach: Reference-to-excitation-to-fluorescence reconvolution. Rev.Sci.Instrum. 64 (1993) 3413-3424

Bevington P. V. and Robinson D. K., Data reduction and error analysis for the physical sciences, Third edition, Mc Graw Hill, 2003

Brochon, J.C. Methods Enzymol. 240 (1994) 262-311

Bryan RK. 1990. Maximum-Entropy Analysis of Oversampled Data Problems. European Biophysics Journal 18 (1990) 165-174.

Teaching methods -
Last update: VECER/MFF.CUNI.CZ (16.05.2008)


Syllabus -
Last update: T_FUUK (14.02.2006)

Fluorescence lifetime measurements in both time and frequency domain

Fluorescence decay curves: basic characteristics; basics of the fluorescence decay analysis

Convolution integral and its approximations; the role of the response function in the analysis

The use of monoexponential references in the fluorescence decay experiments

Response function calculations based on the reference decay measurements

Analytical simulations of the fluorescence decay and anisotropy curves

Parametric analysis of the fluorescence decay data: potential and limitations

The basic principles of the maximum entropy method

Time-resolved fluorescence spectroscopy and 3D peptide studies

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