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Introduction to Protein Structure Studies - NBCM308

Title:	Úvod do studia struktury proteinů
Guaranteed by:	Institute of Physics of Charles University (32-FUUK)
Faculty:	Faculty of Mathematics and Physics
Actual:	from 2011
Semester:	summer
E-Credits:	3
Hours per week, examination:	summer s.:2/0, Ex [HT]
Capacity:	unlimited
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	Czech
Teaching methods:	full-time
Additional information:	http://biomolecules.mff.cuni.cz/Kopecky_Vladimir/courses/

Guarantor:	RNDr. Vladimír Kopecký, Ph.D.
Teacher(s):	RNDr. Kateřina Hofbauerová, Ph.D. RNDr. Vladimír Kopecký, Ph.D.

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Annotation -

The lecture brings in students to the world of proteins and it introduces the basic techniques, theoretical and experimental, used in study of proteins. Emphasis on practical usage of these techniques in protein research will be made. The lecture is useful for students of biophysics, chemical physics, biochemistry and molecular biology.

Last update: T_FUUK (12.05.2005)

Aim of the course -

The goal of the lectures is to give an introduction to practical experimental and theoretical methods which can lead to relevant information on protein structure.

Last update: VLADIK/MFF.CUNI.CZ (23.05.2008)

Course completion requirements -

Oral exam.

Last update: Kopecký Vladimír, RNDr., Ph.D. (30.10.2019)

Literature -

C. Branden, J. Tooze: Introduction to protein structure, Garland Publishing, New York 1991.

H. A. Havel (ed.): Spectroscopic methods for determining protein structure in solution, VCH Publishers, New York 1996.

A. A. Rees, M. J. E. Sternberg, R. Wetzel (eds.): Protein engineering - a practical approach, Oxford University Press, Oxford 1992.

M. J. E. Sternberg (ed.): Protein structure prediction - a practical approach, Oxford University Press, Oxford 1996.

D. R. Westhead, J. H. Parish, R. M. Twyman: Bioinformatics, BIOS Scientific Publishers, Oxford 2002.

Last update: VLADIK/MFF.CUNI.CZ (23.05.2008)

Teaching methods -

Lecture

Last update: VLADIK/MFF.CUNI.CZ (23.05.2008)

Requirements to the exam -

Oral exam.

Last update: Kopecký Vladimír, RNDr., Ph.D. (30.10.2019)

Syllabus -

Primary, secondary, tertiary and quaternary structure of proteins. Protein motives and domains. Structure and function of important types of proteins (e.g. proteins bind DNA or nucleotides, viral or membrane proteins, etc.). Biochemical methods used in study of proteins. Mass spectroscopy of proteins. Usage of circular dichroism, fluorescence spectroscopy, UV-VIS spectroscopy, dynamic light scattering spectroscopy, Raman nonrezonance and resonance spectroscopy, infrared spectroscopy and vibrational circular dichroism in study of proteins. Computer databases useful in study of proteins. Computer modeling of proteins. Assignment of protein structure by means of NMR and X-ray analysis.

Last update: T_FUUK (12.05.2005)