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Course, academic year 2018/2019
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Molecular Mechanisms of Membrane Transport - NBCM304
Title in English: Molekulární mechanismy membránového transportu
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2014
Semester: summer
E-Credits: 3
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: not taught
Language: Czech
Teaching methods: full-time
Guarantor: RNDr. Eva Urbánková, Ph.D.
RNDr. Roman Chaloupka, Ph.D.
Classification: Physics > Biophysics and Chemical Physics
Annotation -
Last update: T_FUUK (28.04.2008)
Membrane transport, membrane transport proteins. Methods of the study of their structure and function. Channels, transporters, antiport, symport, active transport. Important families of membrane transport proteins, focus on several examples. Known mechanisms of the membrane transport.
Aim of the course -
Last update: T_FUUK (12.05.2008)

Study of membrane transport, membrane transport proteins and relevant experimental methods.

Literature -
Last update: URBANKOV/MFF.CUNI.CZ (11.02.2009)

Books:

  • Kotyk A., Janáček K. and Koryta J. (1988) Biophysical Chemistry of Membrane Function (Chichester: Wiley)
  • Yeagle, P.L. (2004) The Structure of Biological Membranes (CRC)
  • Nicholls, D.G, S. J. (2002) Bioenergetics 3 (Academic press)
  • Tamm, L.K. (2005) Protein-lipid interactions: From membrane domains to cellular networks (Wiley-Vch Verlag), Weinheim, Germany
  • White, S.H. (1994) Membrane protein structure: Experimental approaches (Oxford University press)
  • Van Winkle, L.J. (1995) Biomembrane Transport (Academic press)
  • Hille,B. (2001) Ion channels of excitable membranes (Sinauer Associtates, Inc.)
  • Molleman,A. (2003) Patch clamping (John Wiley & sons, LTD)
  • Creighton,T.E. (1993) Proteins (W.H. Freeman and company)
  • B. Alberts, A. Johnson, J. Lewis, M. Raff, K. Roberts, P. Walter (2002) Molecular Biology of the Cell (Garland Science)

Articles:

  • Marsh,D.: Peptide models for membrane channels (1996) Biochem. J. 315: 345-361
  • Spencer,R.H., Rees,D.C.: The a-helix and the organization and gating of channels (2002) Annu. Rev. Biophys. Biomol. Struct. 31: 207-33
  • Wimley,W.C.: The versatile b-barrel memrane protein (2003) Curr. Opin. Struct. Biol. 13: 404-411
  • MacKinnon,R.: Potassium channels (2003) FEBS Lett. 555: 62-65
  • Abramson,J., Kaback,H.R., Iwata,S.: Structural comparison of lactose permease and the glycerol-3-phosphate antiporter: members of the major facilitator superfamily (2004) Curr.Opin. Struct. Biol. 14: 413-419
  • Hunte,C., Screpanti,E., Venturi,M., Rimon,A., Padan,E., Michel,H.: Structure of a Na+/H+ antiporter and insights into mechanism of action and regulation by pH (2005) Nature 435: 1197-1202
  • White,S.H.: Membrane protein folding and stability: physical principles (1999) Annu. Rev. Biophys. Biomol. Struct. 28: 319-65
  • Arechaga,I., Jones,P.C.: The rotor in the membrane of the ATP synthase and relatives (2001) FEBS Lett. 494: 1-5
  • Apell,H.J.: Structure-function relationship in P-type ATPases - a biophysical approach (2003) Rev. Physiol. Biochem. Pharmacol 150: 1-35
  • Schultz,B.E., Chan,S.I.: Structures and proton-pumping strategies of mitochondrial respiratory enzymes (2001) Annu. Rev. Biophys. Biomol. Struct. 30: 23-65

WWW pages:

  • www.tcdb.org
  • http://pdb.rcsb.org/

Teaching methods -
Last update: T_FUUK (12.05.2008)

lecture

Syllabus -
Last update: T_FUUK (28.04.2008)

1. Lipid (bio)membrane. Membrane transport - ions, nonpolar vs. polar molecules, macromolecules. unspecific permeation, active and passive transport. Thermodynamic and kinetic aspects. Membrane potential.

2. Methods of study of membrane transport. Passive transport - unspecific permeation, facilitated diffusion. Channels, transporters. Oligopeptides as a model of membrane proteins.

3. Protein structure (focus on membrane proteins), experimental methods and prediction of protein structure.

4. Theoretical description of transport processes.

5. Channels - peptide channels, toxins, gated selective channels.

6. Secondary active transporters.

7. Primary active transport.

8. Transport of ions (P-, V- and F- type ATPases), ABC pumps.

9. Active transport coupled with redox reaction - proteins of respiratory chain.

10. Protein transport, vesicular transport

Membrane-protein interactions, folding of membrane proteins.

 
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