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Course, academic year 2023/2024
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Advanced methods of optical spectroscopy - NBCM179
Title: Pokročilé metody optické spektroskopie
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2020
Semester: summer
E-Credits: 4
Hours per week, examination: summer s.:3/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: doc. RNDr. Peter Mojzeš, CSc.
doc. RNDr. Jakub Pšenčík, Ph.D.
doc. RNDr. Roman Dědic, Ph.D.
Annotation -
Last update: prof. RNDr. Marek Procházka, Ph.D. (15.05.2020)
Advanced methods and instrumentation of optical spectroscopy used in biophysics and chemical physics.
Course completion requirements -
Last update: doc. RNDr. Jakub Pšenčík, Ph.D. (14.05.2020)

Oral exam in the extent of the syllabus

Literature -
Last update: prof. RNDr. Marek Procházka, Ph.D. (30.04.2019)

[1] Atkins, P. W. a Julio De Paula. Fyzikální chemie. Czech Edition. Vysoká

škola chemicko-technologická v Praze, Praha 2013. ISBN 978-80-7080-830-6.

[2] Prosser V. a kolektiv. Experimentální metody biofyziky. Academia, Praha

1989. ISBN 80-200-0059-3.

[3] Murphy D. B., Davidson M. W. Fundamentals of Light Microscopy and

Electronic Imaging. Wiley-Blackwell, New York 2012. ISBN: 978-1-118-38293-6.

[4] Eaton P., West P. Atomic Force Microscopy. Oxford University Press,

Oxford 2010. ISBN: 978-0-19-957045-4

[5] Drexler W., Fujimoto J.G. Optical Coherence Tomography. Springer-Verlag,

Berlin 2008. ISBN: 978-3540775492.

Syllabus -
Last update: doc. RNDr. Jakub Pšenčík, Ph.D. (14.05.2020)

Advanced imaging methods (FLIM, polarized and spectral FLIM, spectral imaging).

Time-resolved frequency domain fluorescence spectroscopy, FCS.

Fluorescence, confocal, multiphoton and supercompression microscopy.

Nonlinear methods of Raman scattering (HRS, SRS, CARS), Raman optical activity (ROA).

Advanced techniques of Raman spectroscopy (SERS, CRM, DCDR).

Generation and characterization of femtosecond pulses.

Fundamentals of 2DES spectroscopy.

Nonlinear optical phenomena and their use in optical spectroscopy (parametric generation, generation of higher harmonics, Kerr and Pockels effect.

High spectral resolution methods. Low temperature spectroscopy.

Relaxation processes, transverse and longitudinal relaxation time, homogeneous width and lifetime, spectra in a solid matrix.

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