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Course, academic year 2023/2024
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Experimental methods of biophysics and chemical physics I - NBCM177
Title: Experimentální metody biofyziky a chemické fyziky I
Guaranteed by: Department of Chemical Physics and Optics (32-KCHFO)
Faculty: Faculty of Mathematics and Physics
Actual: from 2020
Semester: winter
E-Credits: 6
Hours per week, examination: winter s.:4/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: doc. RNDr. Jakub Pšenčík, Ph.D.
doc. RNDr. Peter Mojzeš, CSc.
prof. RNDr. Petr Heřman, CSc.
Is interchangeable with: NBCM113
Annotation -
Last update: prof. RNDr. Marek Procházka, Ph.D. (08.06.2020)
Basics of optical spectroscopy, magnetic resonance and rentgen difraction methods used in biophysics and chemical physics.
Course completion requirements -
Last update: doc. RNDr. Jakub Pšenčík, Ph.D. (14.05.2020)

Oral exam in the extent of the syllabus

Literature -
Last update: prof. RNDr. Marek Procházka, Ph.D. (30.04.2019)

[1] Atkins, P. W. a Julio De Paula. Fyzikální chemie. Czech Edition. Vysoká

škola chemicko-technologická v Praze, Praha 2013. ISBN 978-80-7080-830-6.

[2] Prosser V. a kolektiv. Experimentální metody biofyziky. Academia, Praha

1989. ISBN 80-200-0059-3.

Syllabus -
Last update: doc. RNDr. Jakub Pšenčík, Ph.D. (14.05.2020)

Sources, detectors and spectrum analyzers in optical spectroscopy

Interaction of optical radiation with an isolated molecule. Dipole approximation

Selection rules for electronic, vibrational and rotational optical transitions.

Methods and applications of electron absorption spectroscopy. Excitation and sounding method.

Use of polarized radiation and its analysis in optical spectroscopy. Linear and circular dichroism.

Methods and applications of vibrational absorption spectroscopy.

Methods of elastic, dynamic and Brillouin scattering and their use.

Raman scattering, methods of measurement and use.

Principles and basic concepts of luminescence (types of luminescence, Jablonsky diagram, kinetics, quantum yield, lifetimes, Franck-Condon principle).

Influence of intermolecular interactions on luminescence parameters (influence of environment, resonant energy transfer, emission quenching).

Emission anisotropy

Measurement of stationary and time-resolved luminescence.

Phosphorescence, single-molecular spectroscopy. Influence of interaction with the environment on the width of the spectral line.

Nuclear magnetic resonance: principle, experimental setup, excitation and signal detection, basic pulse sequences.

High resolution NMR of organic substances in liquids: interpretation of spectra.

Electron paramagnetic resonance: principle, experimental setup, application.

Scattering and diffraction of X-rays, electrons and neutrons.

Principles of basic diffraction methods. Symmetry and structure of crystals and their determination from the diffraction pattern.

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