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2015_CSCF.SupRes.pdf | Superresoluční mikroskopie - článek v Čes. čas. fyz. 2015 | prof. RNDr. Jaromír Plášek, CSc. |
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2015_PMFA.SupRes.pdf | Superresoluční mikroskopie - článek v Pokrocích MFA 2015 | prof. RNDr. Jaromír Plášek, CSc. |
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Dielectric properties of organic compounds. Complex permittivity, mechanisms of polarization, theory of the static permittivity. Interpretation of the frequency-dependent measurements (Debye's equations), temperature dependencies of the relaxation times. Spectrometers for dielectric measurements, selected applications of the dielectric measurements. Transport of the ions through biological membrane. Principles of optical microscopy. Basic concepts: resolving power of the optical microscope. Overview of the methods and their principles. Light field, dark field, phase contrast, anoptrale microscopy, polarized-light microscopy, Nomarsky's method, Hoffman's modulation contrast, fluorescence microscopy. Videomicroscopy and computer-assisted treatment of image. Confocal microscope, near-field microscope. Special techniques: FRAP, microspectrofluorimetry. Confrontation of the microscopy with the other optical methods used for study of the cell morphology: light scattering, flow-cytometry.
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)
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This course aims to provide students of biophysics with a basic overview of experimental methods based on dielectric, conductive and electrochemical properties of biomolecules and biological structures. The course comprises an introduction to use of advanced methods of optical microscopy in biophysics, as well. Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)
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Oral exam. Last update: Procházka Marek, prof. RNDr., Ph.D. (14.05.2020)
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[1] Atkins, P. W. a Julio De Paula. Fyzikální chemie. Czech Edition. Vysoká škola chemicko-technologická v Praze, Praha 2013. ISBN 978-80-7080-830-6. [2] Prosser V. a kolektiv. Experimentální metody biofyziky. Academia, Praha 1989. ISBN 80-200-0059-3. [3] Murphy D. B., Davidson M. W. Fundamentals of Light Microscopy and Electronic Imaging. Wiley-Blackwell, New York 2012. ISBN: 978-1-118-38293-6. [4] Eaton P., West P. Atomic Force Microscopy. Oxford University Press, Oxford 2010. ISBN: 978-0-19-957045-4 [5] Drexler W., Fujimoto J.G. Optical Coherence Tomography. Springer-Verlag, Berlin 2008. ISBN: 978-3540775492. Last update: Procházka Marek, prof. RNDr., Ph.D. (30.04.2019)
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Lecture Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)
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Attendance at lectures. Last update: Procházka Marek, prof. RNDr., Ph.D. (14.05.2020)
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Basic concepts, resolution of optical microscope Overview of imaging methods and their principles Light field, dark field, phase contrast, anoptral microscopy Polarized light microscopy, Nomarsky method, Hoffman modulation contrast Fluorescence microscopy Videomicroscopy and computer image processing Confocal microscope, near-field microscope Special microscopic techniques, FRAP, microspectrofluorimetry Comparison of microscopy with other optical methods of studying cell morphology Current cytometry Biomolecular imaging techniques Raman and IR microscopy TERS and SERS imaging techniques Near field optical scanning microscopy (SNOM) Atomic Force Microscopy (AFM) Mass spectroscopy imaging methods (nano-SIMS) Acoustic imaging methods (ultrasound, sonar) Optical coherence tomography (OCT) Interferometric scattering microscopy (iSCAT) Positron emission tomography (PET, HRRT PET) Methods of fluorescence microscopy with high spatial resolution Last update: Procházka Marek, prof. RNDr., Ph.D. (14.05.2020)
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