SubjectsSubjects(version: 945)
Course, academic year 2023/2024
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Optical Microscopy and Selected Imaging Techniques in Biophysics - NBCM114
Title: Optická mikroskopie a vybrané biofyzikální zobrazovací techniky
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2020
Semester: summer
E-Credits: 3
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: doc. RNDr. Peter Mojzeš, CSc.
Classification: Physics > Biophysics and Chemical Physics
Files Comments Added by
download 2015_CSCF.SupRes.pdf Superresoluční mikroskopie - článek v Čes. čas. fyz. 2015 prof. RNDr. Jaromír Plášek, CSc.
download 2015_PMFA.SupRes.pdf Superresoluční mikroskopie - článek v Pokrocích MFA 2015 prof. RNDr. Jaromír Plášek, CSc.
Annotation -
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)
Dielectric properties of organic compounds. Complex permittivity, mechanisms of polarization, theory of the static permittivity. Interpretation of the frequency-dependent measurements (Debye's equations), temperature dependencies of the relaxation times. Spectrometers for dielectric measurements, selected applications of the dielectric measurements. Transport of the ions through biological membrane. Principles of optical microscopy. Basic concepts: resolving power of the optical microscope. Overview of the methods and their principles. Light field, dark field, phase contrast, anoptrale microscopy, polarized-light microscopy, Nomarsky's method, Hoffman's modulation contrast, fluorescence microscopy. Videomicroscopy and computer-assisted treatment of image. Confocal microscope, near-field microscope. Special techniques: FRAP, microspectrofluorimetry. Confrontation of the microscopy with the other optical methods used for study of the cell morphology: light scattering, flow-cytometry.
Aim of the course -
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)

This course aims to provide students of biophysics with a basic overview of experimental methods based on dielectric, conductive and electrochemical properties of biomolecules and biological structures. The course comprises an introduction to use of advanced methods of optical microscopy in biophysics, as well.

Course completion requirements -
Last update: prof. RNDr. Marek Procházka, Ph.D. (14.05.2020)

Oral exam.

Literature -
Last update: prof. RNDr. Marek Procházka, Ph.D. (30.04.2019)

[1] Atkins, P. W. a Julio De Paula. Fyzikální chemie. Czech Edition. Vysoká

škola chemicko-technologická v Praze, Praha 2013. ISBN 978-80-7080-830-6.

[2] Prosser V. a kolektiv. Experimentální metody biofyziky. Academia, Praha

1989. ISBN 80-200-0059-3.

[3] Murphy D. B., Davidson M. W. Fundamentals of Light Microscopy and

Electronic Imaging. Wiley-Blackwell, New York 2012. ISBN: 978-1-118-38293-6.

[4] Eaton P., West P. Atomic Force Microscopy. Oxford University Press,

Oxford 2010. ISBN: 978-0-19-957045-4

[5] Drexler W., Fujimoto J.G. Optical Coherence Tomography. Springer-Verlag,

Berlin 2008. ISBN: 978-3540775492.

Teaching methods -
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)


Requirements to the exam -
Last update: prof. RNDr. Marek Procházka, Ph.D. (14.05.2020)

Attendance at lectures.

Syllabus -
Last update: prof. RNDr. Marek Procházka, Ph.D. (14.05.2020)

Basic concepts, resolution of optical microscope

Overview of imaging methods and their principles

Light field, dark field, phase contrast, anoptral microscopy

Polarized light microscopy, Nomarsky method, Hoffman modulation contrast

Fluorescence microscopy

Videomicroscopy and computer image processing

Confocal microscope, near-field microscope

Special microscopic techniques, FRAP, microspectrofluorimetry

Comparison of microscopy with other optical methods of studying cell morphology

Current cytometry

Biomolecular imaging techniques

Raman and IR microscopy

TERS and SERS imaging techniques

Near field optical scanning microscopy (SNOM)

Atomic Force Microscopy (AFM)

Mass spectroscopy imaging methods (nano-SIMS)

Acoustic imaging methods (ultrasound, sonar)

Optical coherence tomography (OCT)

Interferometric scattering microscopy (iSCAT)

Positron emission tomography (PET, HRRT PET)

Methods of fluorescence microscopy with high spatial resolution

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