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Course, academic year 2022/2023
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Practical Course in Experimental Methods of Biophysics and Chemical Physics II - NBCM103
Title: Praktikum z experimentálních metod biofyziky a chemické fyziky II
Guaranteed by: Department of Chemical Physics and Optics (32-KCHFO)
Faculty: Faculty of Mathematics and Physics
Actual: from 2020
Semester: summer
E-Credits: 7
Hours per week, examination: summer s.:0/5, MC [HT]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Guarantor: doc. RNDr. Jakub Pšenčík, Ph.D.
prof. RNDr. Marek Procházka, Ph.D.
prof. RNDr. Jan Hála, DrSc.
Classification: Physics > Biophysics and Chemical Physics
Annotation -
Last update: prof. RNDr. Marek Procházka, Ph.D. (12.05.2020)
Practicum of experimental methods of biophysics and chemical physics II
Aim of the course -
Last update: prof. RNDr. Marek Procházka, Ph.D. (12.05.2020)

To familiarize students in practise with advanced experimental techniques.

Course completion requirements -
Last update: prof. RNDr. Marek Procházka, Ph.D. (12.05.2020)

The course is completed by a graded course-unit credit which is awarded on the following conditions:

1. Completion of all 10 tasks by working group.

2. Personal participation in all practical exercises with one absence allowed.

3. Submission of protocols from all tasks, where required (applies to 8 tasks),

and their evaluation by the leaders at least "good". In case of evaluation the degree "failed" allows students to revise the protocol.

The resulting classification is determined as the arithmetic mean of the protocol marks.

Given the nature of the subject, corrective attempts to grant credit are not possible.

Teaching methods -
Last update: prof. RNDr. Marek Procházka, Ph.D. (12.05.2020)

Practical laboratory training.

Syllabus -
Last update: prof. RNDr. Marek Procházka, Ph.D. (12.05.2020)

List of themes:

1. Detection of organic substances (hydrocarbons, alcohols) in the gas phase using an optical sensor based on porous silicon (photoluminescence quenching).

2. Study of acid-base equilibria using Raman spectroscopy.

3. Cultivation of cell cultures and their characterization.

4. Isolation of proteins from natural sources.

5. Nanomaterials and their characterization using DLS and zetapotential.

6. Measurement of translational diffusion by NMR.

7. Spectroscopic detection of singlet oxygen.

8. Determination of thermodynamic parameters of thermally induced acid-base transition by absorption spectroscopy.

9. Electron circular dichroism.

10. High pressure liquid chromatography (HPLC).

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