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Course, academic year 2018/2019
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Yeast Biology - NBCM024
Title in English: Biologie kvasinek
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2007
Semester: summer
E-Credits: 3
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Guarantor: doc. RNDr. Dana Gášková, CSc.
Class: Fyzika
Classification: Physics > Biophysics and Chemical Physics
Annotation -
Last update: T_FUUK (17.05.2001)
Yeast biology Yeast morphology, growth and reproduction, structure of the yeast cell, chemical composition, cell cycle, metabolism, the yeast killer system, pathogenic yeasts, industrially utilized yeasts.
Aim of the course -
Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

Introduction in Yeast Biology

Literature -
Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

A. Kocková-Kratochvílová: Kvasinky a kvasinkové organismy, SNTL, Praha, 1982. A. Kocková-Kratochvílová: Taxonómia kvasinek a kvasinkovitých mikroorganizmov, ALFA, Bratislava, 1990. D. Vraná (Ed.): Kvasinky ve výzkumu a praxi, Academia Praha, 1986. O. Bendová, M. Kahler: Pivovarské kvasinky, SNTL, Praha, 1981.

A. Kocková-Kratochvílová: Kvasinky a kvasinkové organismy, SNTL, Praha, 1982

A. Kocková-Kratochvílová: Taxonómia kvasinek a kvasinkovitých mikroorganizmov, ALFA, Bratislava, 1990

D. Vraná (Ed.): Kvasinky ve výzkumu a praxi, Academia Praha, 1986

O. Bendová, M. Kahler: Pivovarské kvasinky, SNTL, Praha, 1981

Teaching methods -
Last update: GASKOVA/MFF.CUNI.CZ (14.05.2008)

course (facultative)

Syllabus -
Last update: T_FUUK (24.05.2004)

Definition of yeast: Importance and use.

Yeast morphology. Cell morphology ? size and shape, measuring cells, effect of

external factors on cell shape changes, special cells (blastospores, ballistospores,

etc.). Morphology of yeast culture ? growth in a liquid and on a solid medium, types

of budding, single-cell colonies, giant colonies.

Yeast growth and reproduction: Basic yeast nutrition, growth substances, culture

growth ? growth curve (lag phase, exponential, diauxic, post-diauxic, stationary

phase, death phase), growth rate (generation time), synchronized growth, effect of

temperature, effect of inoculum size.

Yeast taxonomy: System according to Kocková-Kratochvílová, key for genus

determination, identification of the most important species and their groups.

Yeast cell structure. Isolation of cell components, cell wall ? structure, scars (types

and staining), protoplasts, induced fusion of protoplasts; membrane ? membrane

systems, secretion pathway, endoplasmic reticulum, Golgi apparatus, secretion

vesicles, modification of proteins during the pathway; vacuole ? function; ribosomes

? cytoplasmic, mitochondrial; cytoskeleton ? methods of study, components,

cytoskeletal functions ? shape changes and motion of organelles; mitochondria ?

shapes, size, structure; nucleus ? structure; cytosol.

Chemical composition of cells: Cell dry weight, inorganic composition, intercellular

and intracellular water, water content of individual organelles, organic cell

components ? nucleic acids and proteins, cell wall polysaccharides (mannans,

glucans, chitin), intracellular saccharides, lipids, sterols, vitamins.

Yeast cell cycle: Cell cycle of Saccharomyces cerevisiae, gene control of cell cycle.

Yeast cell mating: The course of mating, a- and á-cell types, homothallism,

heterothallism, agglutinins.

Sporulation: Initiation of sporulation, sporulation stages, spore composition, mutants.

Metabolism: Requirements of yeast cells for water, temperature, oxygen ? selective

media; transport of substances across the cell wall and cell membrane ? saccharides,

amino acids, ions, peptides, glucose uptake ? regulation of glycolysis; types of yeast

ATPases ? plasma membrane, vacuolar, mitochondrial; catabolite repression ?

hypothesis about its mechanism; energy reserves.

Killer system in yeast.

Pathogenic yeasts.

Industrial yeasts: Requirements for suitable properties, methods of improvement, use

of cell fusion methods and genetic engineering.

 
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