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Course, academic year 2018/2019
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Importance and Functions of Metal Ions in Biological Systems - NBCM023
Title in English: Význam a funkce kovových iontů v biologických systémech
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2001
Semester: winter
E-Credits: 3
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Guarantor: doc. RNDr. Peter Mojzeš, CSc.
RNDr. Jana Zachová, CSc.
Class: Fyzika
Classification: Physics > Biophysics and Chemical Physics
Annotation -
Last update: MOJZES/MFF.CUNI.CZ (12.01.2010)
Inorganic elements in living systems, their occurrence and function. Essentiality and toxicity of metals. Complex ions of transient metals. Interactions of the metals with porphyrins, nucleic acids and proteins. Metabolism of the most important metals (Fe, Cu, Zn, Ni) and the most important enzymes containing trace elements. Chemotherapeutics containing some non-essential metals.
Aim of the course -
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)

The aim of the subject is to provide students of biophysics with more thorough understanding of the role of inorganic elements, notably metal cations, in living systems.

Course completion requirements - Czech
Last update: doc. RNDr. Peter Mojzeš, CSc. (10.10.2017)

Účast na přednášce. Ústní zkouška.

Literature -
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)

Kaim W., Schwederski B.: Bioinorganic Chemistry: Inorganic Element in the Chemistry of Life, John Wiley, Chichester, 1994.

Lippard S.J., Berg J.M.: Principles of Bioinorganic Chemistry, University Science Books, Mill Valley, 1994.

Bertini I., Gray H.B., Stiefel E.I., Valentine J.S.: Biological Inorganic Chemistry, University Science Books, Sausalito, 2007.

Teaching methods -
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)

Lecture

Syllabus -
Last update: MOJZES/MFF.CUNI.CZ (23.05.2008)

1. Inorganic elements in living systems, their occurrence and roles. Metal cations and their significance. Toxicity. Essential and toxic metals. Biominerals. History and current investigation of inorganic elements in biological systems.

2. Fundamentals of coordination chemistry with respect to bioinorganic applications. History of coordination chemistry and basic ideas. Central atom. Ligand. Coordination bond and properties of coordination compounds. Crystal field theory.

3. Molecular orbitals and ligand field theory. Stability of complex ions. Symmetry of coordination complexes. Effect of ligands on energy and spin of central atom electrons. Spectrochemical series. The most important biological ligands of metal cations.

4. Tetrapyrroles as macrocyclic ligands. Axial ligands and their effect on electronic structure, conformation and vibrational states of metalloporphyrins. Effect of central atom on metalloporphyrin-nucleic acids interactions.

5. Interactions of metal cations with nucleic acids. Nucleosides and nucleotides as ligands of transient metals. Significance of metal cations for structural properties and conformational stability of nucleic acids. Role of transient metals in B-Z conformational transition of nucleic acids.

6. Interactions of metal cations with proteins. Aminoacids as ligands of transient metals. Transient metals as components of active centers of enzymes. Cations and stability of proteins.

7. Metabolism of iron. Redox states of iron, solubility and biological role. Transport and storage: transferrin, ferritin, hemosiderin. Heme enzymes: cytochromes, peroxidases. Nonheme iron proteins: FeS-proteins, ribonucleotide reductase, methan monooxygenase.

8. Copper proteins as alternative to biological iron. Blue copper centers, oxidoreductases, cytochrome-c-oxidase, superoxide dismutase.

9. Zinc and its structural and gene-regulatory functions. Zinc enzymes, catalysis of hydrolyse and condensation. Carboanhydrases, alcoholdehydrogenase and related enzymes. Zinc fingers and other zinc regulators. Methalothionein. Zinc-containing DNA-repairing systems.

10. Nickel enzymes. Cancerogenity of nickel, Urease, hydrogenase and related enzymes.

11. Chemotherapy by compounds containing nonessential metals. History. Platinum anticancer drugs. Gold in antiarthritic agents. Bismuth in therapy of gastro-intestinal diseases. Psycho-modulating effects of lithium.

 
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