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Course, academic year 2018/2019
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Vibrational Spectroscopy in Biophysics - NBCM017
Title in English: Vibrační spektroskopie v biofyzice
Guaranteed by: Institute of Physics of Charles University (32-FUUK)
Faculty: Faculty of Mathematics and Physics
Actual: from 2006
Semester: summer
E-Credits: 9
Hours per week, examination: summer s.:0/6 C [hours/week]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: Czech
Teaching methods: full-time
Additional information:
Guarantor: prof. RNDr. Vladimír Baumruk, DrSc.
doc. RNDr. Peter Mojzeš, CSc.
Classification: Physics > Biophysics and Chemical Physics
Annotation -
Last update: doc. RNDr. Peter Mojzeš, CSc. (04.05.2005)
Theoretical and practical course focused on applications of vibrational spectroscopy (IR and Raman) in biophysics and biochemistry. Organized as a block (3 weeks) hands-on training out of semester (august/september) in the form of an international "Summer School on Vibrational Spectroscopy in Biophysics and Biochemistry". Schooling in English. Possibility of individual experimental research using sophisticated spectrometers. Convenient for students of the 3rd and 4th year of the specialization biophysics and chemical physics, doctoral students F4 (biophysics, chemical and macromolecular physics), and students of biophysical, chemical and biochemical curricula from other domestic as well as foreign universities.
Aim of the course -
Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)

Students will be acquainted with techniques of vibrational spectroscopy including practical exercises in a spectroscopic laboratory.

Literature - Czech
Last update: doc. RNDr. Peter Mojzeš, CSc. (04.05.2005)

Schrader B.: Infrared and Raman Spectroscopy, VCH, Weinheim, 1995.

Carey P.R.: Biochemical Applications of Raman and Resonance Raman Spectroscopies, Academic Press, New York, 1982.

Teaching methods -
Last update: BAUMRUK/MFF.CUNI.CZ (23.05.2008)

summer school - lectures and practical training in a spectroscopic laboratory

Syllabus -
Last update: doc. RNDr. Peter Mojzeš, CSc. (06.05.2005)

1. Basic principles of vibrational spectroscopy. Classical and quantum-mechanical model. Infrared and Raman spectroscopies. Resonance Raman effect.

2. Advantages and disadvantages of Raman and IR spectroscopies for biophysical, biochemical and biological studies.

3. Experimental vibrational spectroscopy. Basics of Raman and infrared absorption experiment.

4. Advanced techniques of Raman spectroscopy: Surface-enhanced Raman scattering, Raman optical activity.

5. Applications of IR and Raman spectroscopy to study structural properties and interactions of nucleic acids, proteins and porphyrins.

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