SubjectsSubjects(version: 901)
Course, academic year 2021/2022
Therapeutical proteins - MC250P67
Title: Terapeutické proteiny
Czech title: Terapeutické proteiny
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 2
Examination process: summer s.:combined
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Level: specialized
Note: enabled for web enrollment
Guarantor: RNDr. Ondřej Vaněk, Ph.D.
Teacher(s): RNDr. Ondřej Vaněk, Ph.D.
Annotation -
Last update: RNDr. Ondřej Vaněk, Ph.D. (24.01.2018)
Lecture presents the topic of therapeutic proteins as a new branch of biopharmaceuticals gaining growing interest as a tool in diagnostics and treatment of cancer, hormonal disorders and vaccination. It deals mainly with modern techniques of recombinant protein expression (including practical tips) and gives an overview of structural and functional classes of protein therapeuticals with a special regard to new and alternative protein binding scaffolds. A separate chapter is devoted to the protein modification, conjugation and administration issues.
A special interest is given to practical examples - each lecture contains besides the part from the main topic also a part describing one example of a therapeutically used protein, with review of its research, development and current usage.

Lectures are delivered in Czech to Czech students. For Erasmus students the lectures are in English. Depending on the number of students enrolled the course can be arranged as lectures or in another way (colloquium, consultations).
Literature -
Last update: RNDr. Ondřej Vaněk, Ph.D. (03.05.2012)

Biopharmaceutical benchmarks. Walsh G. Nat Biotechnol. 18 (2000): 831-833.

Biopharmaceutical benchmarks 2003. Walsh G. Nat Biotechnol. 21 (2003): 865-870.

Biopharmaceutical benchmarks 2006. Walsh G. Nat Biotechnol. 24 (2006): 769-776.

Biopharmaceutical benchmarks 2010. Walsh G. Nat Biotechnol. 28 (2010): 917-924.

Expression systems. Dyson M. R., Durocher, Y. eds. Bloxham, Scion Publishing Ltd., 2007

Requirements to the exam -
Last update: RNDr. Ondřej Vaněk, Ph.D. (20.04.2021)

The exam will consist of a written part (test) and an oral part (discussion of the test and related questions).

Syllabus -
Last update: RNDr. Jiří Liberda, Ph.D. (11.05.2011)

Introduction to protein therapeuticals: review of current and future market, ways of approval. Modern methods of recombinant protein expression: overview, choosing the right expression host. Cloning with and without restriction endonucleases. Prokaryotic expression systems: pros and cons, news and techniques. Eukaryotic expression systems: yeast, protozoa, plant expression systems and insect cells. Protein production in mammalian cell lines: stable and transient transfection, selection and yield improvement, cell line engineering. Monoclonal antibodies and their recombinant fragments. Hormones, cytokines, other small therapeutics. Recombinant vaccines: boons and risks. New alternative protein binding scaffolds: (not only) alternatives to antibodies. Therapeutic protein modifications: conjugation with polymers, solid phase depot formulation, administration issues of protein solutions, protein radioconjugates.

Entry requirements -
Last update: RNDr. Ondřej Vaněk, Ph.D. (16.02.2012)

Your interest in the lecture topic:-)

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