Proteins - organic chemistry and biochemistry - MC250P28

• Title: Proteiny - organickochemické a biochemické aspekty
• Czech title: Proteiny - organickochemické a biochemické aspekty
• Guaranteed by: Department of Biochemistry (31-250)
• Faculty: Faculty of Science
• Actual: from 2022
• Semester: summer
• E-Credits: 3
• Examination process: summer s.:
• Hours per week, examination: summer s.:2/0, Ex [HT]
• Capacity: unlimited
• Min. number of students: unlimited
• Virtual mobility / capacity: no
• State of the course: taught
• Language: Czech
• Note: enabled for web enrollment
• Guarantor: prof. RNDr. Helena Ryšlavá, CSc.
• Teacher(s): RNDr. Kateřina Bělonožníková, Ph.D.; prof. RNDr. Helena Ryšlavá, CSc.

Annotation

English

Last update: RNDr. Jiří Liberda, Ph.D. (16.12.2009)

Proteins, method of their isolation and characterization, primary structure; affinity technique in the study of binding properties of proteins.
Lectures - in Czech -

Czech

Last update: prof. RNDr. Helena Ryšlavá, CSc. (21.01.2022)

Proteiny: metody jejich isolace a charakterizace, primární struktury proteinů, afinitní techniky pro studium vazebných vlastností proteinů.

Literature

Last update: SRBA (19.08.2002)

Literatura:

Biochemistry: R.H. Garrett, C.M. Grisham, Harcourt Brace College Publishers, Fort WORTH, 1995

Proteins: Structure and Molecular Properties: T.E. Creighton, W.H. Freeman 1992

Protein Purification: Principle and Practice, Springer- Verlag 1982

Requirements to the exam

Last update: RNDr. Jiří Liberda, Ph.D. (26.04.2012)

ústní zkouška v rozsahu sylabu

Syllabus

English

Last update: Mgr. Alice Šonská, Dr. (08.01.2009)

Isolation of proteins: different types of separation techniques. Methods used for characterization of proteins; sources of heterogeneity of protein samples, determination of proteins, Structure of proteins: determination of tertiary structure. Chemical modification of proteins, modification in vivo. Affinity techniques in separation of proteins and in characterization of binding properties of proteins.

References:

Biochemistry: R.H. Garrett, C.M. Grisham, Harcourt Brace College Publishers, Fort WORTH, 1995

Proteins: Structure and Molecular Properties: T.E. Creighton, W.H. Freeman 1992

Protein Purification: Principle and Practice, Springer- Verlag 1982

Prof. M. Tichá a H. Ryšlavá

Czech

Last update: RNDr. Kateřina Bělonožníková, Ph.D. (11.04.2022)

Izolace proteinů: různé typy separačních technik. Metody užívané pro charakterizaci proteinů, zdroje heterogenity izolovaných proteinových preparátů, stanovení proteinů. Struktura proteinů, určení primární struktury. Afinitní techniky využívané pro separaci proteinů a při charakterizaci vazebných vlastností proteinů.