SubjectsSubjects(version: 901)
Course, academic year 2022/2023
  
Biomolecular motion and intracellular transport - MC250P27
Title: Pohyby biomolekul a vnitrobuněčný transport
Czech title: Pohyby biomolekul a vnitrobuněčný transport
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2019
Semester: winter
E-Credits: 2
Examination process: winter s.:
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Note: enabled for web enrollment
Guarantor: RNDr. Marek Ingr, Ph.D.
Teacher(s): RNDr. Marek Ingr, Ph.D.
Opinion survey results   Examination dates   Schedule   
Annotation -
Last update: RNDr. Marek Ingr, Ph.D. (26.02.2018)
The lecture is concerned with the causes and circumstances of the motion of individual objects within eukaryotic cells mediated by special protein molecules and supramolecular systems - cytoskeletal molecular motors. They facilitate targeted transfer of molecules, vesicles and organelles among individual cellular compartments, motion of flagella and cilia, changes of cellular shape, etc. The content of the lecture is oriented on thermodynamic, kinetic and mechanistic explanation of the described phenomena, but also on the description of important applications from cell energetics, cell reproduction, and neurobiology, medical point of view is also taken into the account. Specific research methods of studying molecular motors are briefly discussed.
Lectures are delivered in Czech to Czech students. For Erasmus students the lectures are in English. Depending on the number of students enrolled for the course, it can be arranged as regular or irregular (colloquium, consultations) lectures.
Literature - Czech
Last update: RNDr. Marek Ingr, Ph.D. (25.04.2012)

Schliwa, M. (ed.): Molecular motors. VCH-Wiley, Weinheim, 2003

Alberts a spol.: Základy buněčné biologie. Espero Publishing, Ústí nad Labem

Requirements to the exam - Czech
Last update: RNDr. Marek Ingr, Ph.D. (25.04.2012)

Zkouška je ústní, student si náhodně zvolí dvě otázky, jednu z biologicky a jednu z fyzikálně zaměřené části učiva. Požadována je znalost všech podstatných bodů probíraného učiva v rozsahu přednášky.

Syllabus -
Last update: SIMONAT (25.02.2004)

Cytoskeleton structure, microtubules and microfilaments and their synthesis and decomposition, distribution and orientation in the cell, influence on the cellular shape

Cytoskeletal molecular motors, classes of kinesins, dyneins, and myosins, structure and function

Function of kinesins - intracellular transport of vesicles and orgnelles and its targetting, orientation of movement along cytoskeletal structures, transport of chromosomes during cell division

Function of dyneins - flagelar and ciliary motion of unicellular animals, sperm and epithelial cells

Function of myosins - muscle contraction, transport in the proximity of cell membrane

Comparison of cytoskeletal and membrane molecular motors, transformation of the energy of macroergic bonds of ATP to energy of ion rgadients and vice versa

Principles of molecular motors' function, ATPase activity, power-stroke theory, Brownian motors, noice induced transport

Methods of exploration of molecular motors, single-molecule manipulation, optical-tweezer method, electron microscopy

References:

Schliwa, M. (ed.): Molecular motors. VCH-Wiley, Weinheim, 2003

 
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