SubjectsSubjects(version: 920)
Course, academic year 2022/2023
   Login via CAS
Modern methods in protein research - MC250P21
Title: Moderní metody výzkumu proteinů
Czech title: Moderní metody výzkumu proteinů
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2018
Semester: summer
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: 5
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Note: enabled for web enrollment
Guarantor: RNDr. Petr Man, Ph.D.
Teacher(s): Ing. Aleš Hnízda, Ph.D.
Mgr. Josef Chmelík, Ph.D.
RNDr. Petr Man, Ph.D.
RNDr. Petr Novák, Ph.D.
Annotation -
Last update: RNDr. Petr Man, Ph.D. (09.02.2023)
This lecture deals with the modern methods of protein research. It focuses on structural and affinity proteomics, including methods of chemical cross-linking, oxidative labelling, hydrogen/deuterium exchange and native mass spectrometry. It is also explained how to further process the data using bioinformatic approaches and build or correct structural protein models.
Lectures, student's presentations and essay (exam) are in English.

Lecture room 218 at the dept. of biochemistry, Hlavova 8, 2nd floor -
Literature -
Last update: RNDr. Petr Man, Ph.D. (20.01.2018)

Shan S. Wong - Chemistry of protein conjugation and cross-linking, CRC Press
Greg T. Hermanson - Bioconjugate Techniques, Elsevier
Methods in Enzymology - Biological Mass Spectrometry
T. E. Creighton - Protein Structure, A practical approach, IRL Press
Marc R. Wilkins - Proteome Research, Concept, technology and Application, Springer
John M. Walker - The protein Protocols Handbook, Humana Press
selected titles from  Methods in Molecular Biology Humana Press
vol. 61 - Protein and Peptide Analysis by Mass Spectrometry
vol. 64 - Protein Sequencing Protocols
vol. 146 - Mass spectrometry of proteins and peptides
vol. 194 - Posttranslational Modifications of proteins
vol. 244 - Protein Purification Protocols
vol. 251 - HPLC of Peptides and Proteins
vol. 261 - Protein-Protein Interactions
vol. 367 - Mass Spectrometry Data Analysis in Proteomics
vol. 446 - Post-Translational Modifications of Proteins

Requirements to the exam -
Last update: RNDr. Petr Man, Ph.D. (05.02.2021)

Exam composes of active participation on the journal club (short presentation) and written essay on selected topic. More details will be given at the first lecture.

for consultation please use e-mail : @

Syllabus -
Last update: RNDr. Petr Man, Ph.D. (19.02.2019)

Chemical reactions of proteins, mass spectrometry,  H/D exchange, chemical cross-linking, native mass spectrometry, affinitz mass spectrometry.


Charles University | Information system of Charles University |