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Hemeproteins and metalloproteins - MC250P11
Title: Hemoproteiny a metaloproteiny
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2022
Semester: summer
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: English, Czech
Note: enabled for web enrollment
Guarantor: prof. RNDr. Jiří Hudeček, CSc.
Teacher(s): prof. RNDr. Jiří Hudeček, CSc.
Annotation -
Last update: prof. RNDr. Jiří Hudeček, CSc. (19.01.2018)
Functional and structural aspects of heme- and other metallloproteins. Porphyrins and metalloporphyrins, nomenclature, structure, physicochemical properties, natural abundance, biosynthesis and catabolism). Biochemical oxygen carriers, myoglobin, hemoglobin, their structure, function, regulation, evolution and molecular pathology, non-heme oxygen carriers. Electron transfer in biochemistry, cytochromes, their structure, functional role in respiratory chain and similar electron transfer chains, and in photosynthesis. Cytochrome c, analysis of its structure-function relationships, evolutionary aspects. Heme enzymes, activation of molecular oxygen, P450 proteins, catalases and peroxidases, Nosynthase. Heme sensor proteins and NO carriers. Other reaction of oxygen and its active species, oxygenases and dioxygenases, superoxiddismutases. Open chain tetrapyrrols and porphyrine analogs, bilins, phytochrome, chlorophylls, cobalamine. General considerations of inorganic biochemistry, role of metals in biochemistry.

Lectures - in Czech and/or in English (in case of more than 2 interested students; otherwise the English course takes the form of a "directed reading").
Literature -
Last update: prof. RNDr. Jiří Hudeček, CSc. (16.02.2020)

Berg., J.M., Tymoczko J.L, Stryer L.: Biochemistry. 6th edition, WH Freeman and Co., N. York 2007
Nelson, D.L., Cox M.M.: Lehninger Principles of Biochemistry, 5th Edition, WH Freeman and Co., N. York 2008 

Bertini L, Gray HB, Lippard SJ, Valentine JS: Bioinorganic chemistry. University Science Books, Sausalito 1994.

Adv. Inorg. Chem Vol. 51 (2001) (Sykes, AG, Mauk G., eds) Academic Press

Roat-Malone R.M. Bioinorganic Chemistry, A Short Course, 2nd Ed. , Wiley 2007.

Ghosh A. (ed.) The Smallest Biomolecules. Diatomics and Their Interactions with Heme Proteins. Elsevier 2008.

Ochiai N.: Bioinorganic Chemistry. A Survey. Academic Prass 2008.

Crichton R. R.: Biological Inorganic Chemistry. An Introduction. Elsevier 2008.

D. Rehder: Bioinorganic Chemistry. Oxford University Press 2014.

L. Zhang: Heme Biology: The Secret Life of Heme in Regulating Diverse Biological Processes. World Scientific Publishing Company 2011.



Requirements to the exam -
Last update: prof. RNDr. Jiří Hudeček, CSc. (04.11.2011)

Oral examination, contents see syllabus.

Syllabus -
Last update: SIMONAT (30.04.2002)

1. Porphyrins and metalloporphyrins - nomenclaure, natural abundance, physico-chemical properties, spectroscopy and structure. Analogues and its use in therapy.

2. Hemeproteins - functional classification, common physico-chemical and structural properties.

3. Oxygen carriers and similar binding proteins. Myoglobin (characteristics, functional role of protein chain). Hemoglobin - differences and similaritites to myoglobin. Allostery - phenomenological description and theoretical models, effectors and modulators. Ontogenetic and phylogenetic aspects - fetal hemoglobin, molecular pathology (hemoglobinopathies, anemia, thallassemias) non-mammalian hemoglobins, leghemoglobin. Non-heme oxygen carriers (hemerythrin, hemocyanin). NO carriers - nitrophorin.

4. Electron transfer. Cytochromes, cytochrome c (description, functional and phylogenetic aspects). Electron transfer chains (respiratory chain, electron transfer in photosynthesis).

Other cytochromes and flavocytochromes. FeS proteins.

5. Heme enzymes, oxygen activation. Cytochromes P450, NO synthase. Oxidases, oxygenases. Peroxidases, catalase, superoxidismutases.

6. Heme sensors - sGC, FixL, CooA.

7. Metobolism of heme and iron. Porphyrias, jaundice. Ferritin, transferrin.

8. Porphyrin analogues as coenzymes. Chlorophyll, photosynthetic reaction centers, Phytochrome, open chain tetrapyrroles, phycobillin.

9. General introduction to biochemical importance of metals, detoxication, metallothioneins.

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