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Course, academic year 2022/2023
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Introduction to advanced biochemistry - MC250P02
Title: Úvod do pokročilé biochemie
Czech title: Úvod do pokročilé biochemie
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2020
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: prof. RNDr. Helena Ryšlavá, CSc.
Teacher(s): prof. RNDr. Jiří Hudeček, CSc.
Annotation -
Last update: prof. RNDr. Jiří Hudeček, CSc. (03.10.2022)
The course aims at the consolidation of biochemical knowledge of the students to the extent of allowing the smooth study of the specialized and advanced biochemical lectures. Additionally, its seminar part is based on independent work with literature and Internet-based informational services. The students have to prepare short presentations on given themes.
Lectures and presentations - in Czech -
Literature -
Last update: prof. RNDr. Jiří Hudeček, CSc. (03.10.2022)

Kodíček, M. , Valentová O., Hynek R.: Biochemie, VŠCHT Praha 2015

Nelson, DL, Cox, MM: Lehningers Principles of Biochemistry, 7th Ed. WH Freeman , N. York, 2017

Requirements to the exam -
Last update: prof. RNDr. Jiří Hudeček, CSc. (03.10.2022)

Credit: seminar presentation on the given theme., participation in seminars (80% or more).

Exam: in the written form. Response to questions, of the "miniessay" character. The answers are evaluated both according to the content (completness, absence of errors) and to the formal quality (logic of presentation).

Broader themes:
Living matter, its characteristics, compartmentation, trophic.
Biopolymer structure, basic concepts, hierarchy, comparison of structural types, relationships to biological function.
Proteins, structural types, stability and denaturation, physicochemical properties.
Enzymesy and biocatalysis, classification and nomenclature, inhibition, influece of external factors, analytics and diagnostics.
Metabolism, general principles, reversibility an dregulation, nutrients and their fate in organisms, bioenergetics, importance of oxygen, macroergic compounds.
Mitochondria and respiration, TCA, ATP formation, ROS.
SaCcharides and their metabolism, glycolysis, fermantation, polysaccharides.
Lipids amd their metabolism, structure. importance as energetic reserve.
Metabolism of nitrogen-containing compounds, amino acids, amines, ornithine cycle, detoxication of ammonia, purines and pyrimidines, heme.
Nukleic acids, transfer of genetic information, RNA, DNA types and structures, genetic code, replication, transcription, translation, posttranslational modifications.

Syllabus -
Last update: prof. RNDr. Jiří Hudeček, CSc. (23.10.2019)

Living matter: general characteristics of life, composition, trophic, cell, the importance of compartmentation, biological membranes.

Biopolymers: general principles of structure, the hierarchy of structures, comparison of individual types, physicochemical properties, biochemical functions, structure-activity relationships.

Proteins: structural types, primary, secondary, tertiary and quaternary structures, stability and denaturation, physicochemical properties, composite proteins.


Enzymes and biocatalysis: catalysts, classification of enzymes, activity, its influence by external factors, inhibition, measurement of enzyme activity, use in analytics and diagnostics.

Metabolism: general patterns, reversibility and regulation, energy cycle in the biosphere, photosynthesis, metabolism of heterotrophic organisms and man - nutrients and their fate, integration of metabolic processes, the importance of oxygen, macroergic compounds, secondary metabolites.

Mitochondria and cellular respiration: citrate cycle, respiratory chain, ATP formation, reactive oxygen species, alternative ways of nutrient utilization.

Carbohydrates and their metabolism: carbohydrates as a source of energy in food, polysaccharides - cellulose, starch, glycogen, glycolysis, fermentation processes.

Lipids and their metabolism: lipid structure, the importance of fats as storage substances, degradation and biosynthesis. Nitrogen metabolism: amino acids, amines, urea cycle, purine and pyrimidine bases, haem.

Nucleic acids and transfer of genetic information: structure of RNA and DNA, genetic code, replication, transcription, translation, protein folding and chaperones, post-translational modification of proteins.

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