SubjectsSubjects(version: 978)
Course, academic year 2025/2026
   
Advanced practical course in biochemistry II - MC250C08
Title: Pokročilé praktikum II
Czech title: Pokročilé praktikum II
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2025
Semester: summer
E-Credits: 8
Examination process: summer s.:
Hours per week, examination: summer s.:0/2, C [TS]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
priority enrollment if the course is part of the study plan
Guarantor: RNDr. Ondřej Vaněk, Ph.D.
Teacher(s): RNDr. Asfaw Befekadu, CSc.
prof. RNDr. Pavla Bojarová, Ph.D.
prof. RNDr. Petr Hodek, CSc.
RNDr. Tomáš Koblas, Ph.D.
prof. RNDr. Pavel Souček, CSc.
Ing. Kvido Stříšovský, Ph.D.
doc. RNDr. Miroslav Šulc, Ph.D.
RNDr. Ondřej Vaněk, Ph.D.
RNDr. Lenka Žáková, Ph.D.
Annotation -
The aim of the practical course is to prepare diploma students to write their thesis and pursue their future scientific and research careers. This course is intended for first-year students of the master's program in biochemistry, and admission requires a credit from Advanced Practical Biochemistry (MC250C17E) or Advanced Practical Biochemistry for KATA (MC250C03E). Under the guidance of experienced consultants, students work on fourteen-day projects designed to allow them to try out and practice advanced techniques commonly used in immunochemistry, biochemistry, and molecular biology, such as ELISA, Western blotting, immunoaffinity chromatography, animal premedication, enzyme and protein purification using HPLC, gel filtration, ion exchange, affinity and chelation chromatography, enzyme characterization by measuring their activities and spectral properties, protein mass spectrometry, fluorescence microscopy, PCR techniques, cell culture, DNA sequencing, and microseparation techniques. Considerable attention is paid to developing and strengthening students' independence, particularly in planning experiments and setting work schedules. Students also practice presenting their results as posters and in lectures at a student science conference held after the end of the practical course. Study materials and laboratory manuals are recommended or provided by the consultants of the relevant project. After completing the practical course, students receive a credit, for which they must meet the following conditions: 1. at least 70% attendance, 2. submission and defense of a report containing the results of the project, including their discussion, and 3. presentation of a poster or lecture at a student conference. The practical course syllabus is not available because individual projects are prepared and published shortly before the start of the practical course.
Last update: Vaněk Ondřej, RNDr., Ph.D. (04.02.2026)
Literature -

Literature is not available beforehand, because individual projects in a given year are prepared and published shortly before the start of the practical course. After registering for a given project, the project leader can provide you with the relevant literature.

Last update: Vaněk Ondřej, RNDr., Ph.D. (03.02.2026)
Requirements to the exam -

The conditions for receiving credit are: 1) 100% attendance at the mandatory introductory meeting (where safety training will be provided and organizational and technical matters related to the practical course will be discussed), 2) at least 70% active participation of each student at the workplace in solving the assigned project, 3) submission and defense of a report summarizing the obtained results, methods used, and results achieved (the report must be signed by the project leader), and 4) presentation of a poster and lecture (in pairs) at a conference to be held with the participation of department members and guests after the end of the practical course (probably 13. 5. 2026). The practical course begins with a meeting on 27. 2. 2026 at 2 p.m. in the Advanced Biochemistry Practical Laboratory (room no. 201), where the course's organizational principles will be discussed, and knowledge of laboratory safety rules will be tested (file BOZP_temata.pdf). If you do not attend this introductory meeting, your course registration will be considered invalid, and the project will be canceled.

Last update: Vaněk Ondřej, RNDr., Ph.D. (04.02.2026)
Syllabus -

The practical course syllabus is not available because individual projects are prepared and published shortly before the start of the practical course.

Last update: Vaněk Ondřej, RNDr., Ph.D. (03.02.2026)
Entry requirements -

The prerequisite for participation in the practical course is participation in the introductory meeting, which will be held on 27. 2. 2026 at 2 p.m. in the Advanced Biochemistry Practical Laboratory (room no. 201).

Last update: Vaněk Ondřej, RNDr., Ph.D. (04.02.2026)
 
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