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Course, academic year 2022/2023
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Advanced practical course in biochemistry II - MC250C08
Title: Pokročilé praktikum II
Czech title: Pokročilé praktikum II
Guaranteed by: Department of Biochemistry (31-250)
Faculty: Faculty of Science
Actual: from 2021
Semester: summer
E-Credits: 8
Examination process: summer s.:
Hours per week, examination: summer s.:0/2, C [TS]
Capacity: unlimited
Min. number of students: unlimited
For 4EU+ students: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: prof. RNDr. Petr Hodek, CSc.
Teacher(s): RNDr. Asfaw Befekadu, CSc.
doc. RNDr. Pavla Bojarová, Ph.D.
Mgr. Zbyněk Heger, Ph.D.
prof. RNDr. Petr Hodek, CSc.
RNDr. Tomáš Koblas, Ph.D.
doc. RNDr. Pavel Souček, CSc.
doc. RNDr. Miroslav Šulc, Ph.D.
RNDr. Ondřej Vaněk, Ph.D.
RNDr. Mgr. Lenka Žáková, Ph.D.
Annotation -
Last update: prof. RNDr. Petr Hodek, CSc. (22.02.2019)
The aim of the course is to make students ready for their diploma work and further career. This practical course (laboratories) are arranged for biochemistry students of the 4 th grade. The credit from the Advanced practical course in Biochemistry, Pt. I is required for enrolling student to the course. Students, under a supervision of tutors, work on two-week research projects. The projects are designed to train them in use of various advanced techniques of immunochemistry, biochemistry, and molecular biology: e.g. ELISA, Western blotting, immunoaffinity chromatography, treatment of animals, purification of enzymes and proteins using RP-HPLC, size-exclusion, ion-exchange, affinity and adsorption chromatography, characterization of enzymes by measuring their activities, spectral properties, protein sequencing, PCR technique, cell cultures, DNA sequencing, microseparation techniques. A special attention is paid to student's independence in an experiment design and its time table. Students are trained also in their presentation skills on a student's scientific conference, where they have a lectures or present posters dealing with results of their projects. To all the projects detailed manuals are provided by tutors, whose also suggest appropriate literature (monographs or articles in scientific journals) concerning their project. Students are awarded with credits when they meet the following criteria: 1. at least 70% attendance within the working time, 2. submittion of a result sheet containing all the data obtained including their discussion, 3. presentation of a poster or a lecture at the student's conference. No sylabus of the course is available since projects are designed and posted at least one month before the course starts.
Literature -
Last update: RNDr. Ondřej Vaněk, Ph.D. (12.02.2019)

Literature is not available beforehand, because individual projects in a given year are known only about one month before the practical course when students enroll for the course.

Requirements to the exam -
Last update: prof. RNDr. Petr Hodek, CSc. (09.03.2022)
TEXT je bily To obtain credits, students should: i/ be present at the introductory meeting where the safety rules and the organization of practical course will be present, ii/attend the practical course (at least 70% work hours), iii/submit lab reports (signed by the tutor), and iv/give the lecture based on the obtained results and present a poster at the simulated conference on 24.9.2021. The introductory meeting will be 4.3.2021 from 2 a.m. in the CH2 lecture hall. 
Syllabus -
Last update: RNDr. Jiří Liberda, Ph.D. (18.09.2009)

Sylabus of the practical course is not available since the individual projects are designed and announced always before the course starts.

Entry requirements -
Last update: prof. RNDr. Petr Hodek, CSc. (09.03.2022)

TEXT je bily The prerequisite for participation in the practical course is participation in the introductory meeting, which will be held 4.3.2021 from 2 a.m. in CH2 lecture hall. 

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