SubjectsSubjects(version: 901)
Course, academic year 2022/2023
Analytical Spectrometry - MC230P81
Title: Analytical Spectrometry
Czech title: Analytická Spektrometrie
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2020
Semester: winter
E-Credits: 2
Examination process: winter s.:combined
Hours per week, examination: winter s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: English
Note: enabled for web enrollment
Guarantor: RNDr. Jakub Hraníček, Ph.D.
Teacher(s): RNDr. Jakub Hraníček, Ph.D.
RNDr. Jan Kratzer, Ph.D.
RNDr. Eliška Nováková, Ph.D.
Opinion survey results   Examination dates   Schedule   
Last update: RNDr. Jakub Hraníček, Ph.D. (25.10.2021)
This course is taught in English for Erasmus students and serves as a basic introduction to the spectrometric methods useful in analytical chemistry. Lectures presented in English contain basic information about the following areas: X-ray spectrometry, atomic absorption, and emission techniques, molecular absorption in UV, VIS, and IR regions, molecular fluorescence, and nuclear magnetic resonance. Individual topics are briefly characterized in the syllabus. All topics are completed with theoretical principle, instrumentation, and practical utilization, mainly in analytical chemistry.

During epidemiological measures (COVID-19): This subject is taught in an online form (Google Meet).

RNDr. Jakub Hraníček, Ph.D.;
RNDr. Jan Kratzer, Ph.D.;
RNDr. Eliška Nováková, Ph.D.:

This course starts on Tuesday, October 19th, 2021.


19.10. Information meeting, organization, Introduction to the Analytical Spectrometry (JH)
26.10. X-ray Spectrometry (JH)
2.11. Atomic Absorption Spectrometry (JK)
9.11. Atomic Emission and Fluorescence Spectrometry (JK)
16.11. Inductively Coupled Plasma Mass Spectrometry + approaches to sample treatment (JK)
(Excursion to the Institute of Analytical Chemistry of the CAS, v. v. i.)
23.11. UV-VIS Spectrometry (EN)
30.11. Molecular Fluorescence Spectrometry + methods based on the radiation scattering (EN)
7.12. Nuclear Magnetic Resonance Spectrometry (EN)
14.12. Infrared Spectrometry (JH)
21.12. Students presentation (JH, EN, JK)
4.1. 2022 Written examination (JH)

Last update: RNDr. Jakub Hraníček, Ph.D. (29.09.2021)

Presentation from individual lectures are main literature sources for examination.


Extended information are avaible here.

Handbook of High-resolution Spectroscopy. Wiley 2011

Laser Chemistry. Spectroscopy, Dynamics and Applications. Wiley 2007

Surface Enhanced Raman Spectroscopy. Analytical, Bioanalytical and Life Science Application. Wiley 2010 

Modern Spectroscopy. 4th Ed. Wiley 2004

Requirements to the exam
Last update: RNDr. Jakub Hraníček, Ph.D. (29.09.2021)

a) Short oral presentation about the selected topic.

b) Written examination about the topics being taught. 

Last update: RNDr. Jakub Hraníček, Ph.D. (29.09.2021)

1. Introduction to the Analytical Spectrometry (basic relations, analysis)

2. X-ray Spectrometry (principles, practical analysis)

3. Atomic Emission Spectrometry

4. Atomic Absorption Spectrometry

5. Atomic Fluorescence Spectrometry

6. Molecular Spectrometry in UV/VIS regions

7. Molecular Fluorescence

8. Infrared and Raman Spectrometry

9. Nuclear Magnetic Resonance

10. Other methods of Analytical Spectrometry

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