SubjectsSubjects(version: 901)
Course, academic year 2022/2023
Advances in Analytical Spectrometric Methods - MC230P65
Title: Advances in Analytical Spectrometric Methods
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2022
Semester: summer
E-Credits: 3
Examination process: summer s.:written
Hours per week, examination: summer s.:2/0 Ex [hours/week]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: English
Level: specialized
Note: enabled for web enrollment
Guarantor: RNDr. Jan Kratzer, Ph.D.
Opinion survey results   Examination dates   Schedule   
Annotation -
Last update: RNDr. Jan Kratzer, Ph.D. (10.02.2022)
This course is taught in English for Erasmus and PhD students. New trends and techniques in the fields of atomic and molecular spectroscopy and mass spectrometry are discussed including their applications. Also advanced approaches to sample preparation and preconcentration are presented. The series of lectures is given by experienced researchers from Czech universities and Czech Academy of Sciences. Basic knowledge of fundamental spectroscopic techniques is expected. Active participation of the students is expected with maximum absence of 20%.

The course starts on Wednesday 23 February 2022.

Preliminary programme for summer semester 2022 (dates are still subject to change):

Date Lecturer Topic Institution
23.2. RNDr. Jan Kratzer, Ph.D. Introduction to atomic, molecular and mass spectrometry IAC CAS
2.3. RNDr. Tomáš Matoušek, Ph.D. Elemental speciation analysis IAC CAS
9.3. Ing. Adam Sklenář Chiroptical spectroscopy for structural analysis of biomolecules I IOCB CAS
16.3. Ing. Věra Schrenková Chiroptical spectroscopy for structural analysis of biomolecules II IOCB CAS
23.3. RNDr. Václav Červený, Ph.D. Spectrometric analysis without sample preparation FS CU
30.3. RNDr. Jan Kratzer, Ph.D. Preconcentration techniques for trace element analysis and atomic spectrometry IAC CAS
6.4. Ing. Antonín Kaňa, Ph.D. From proof of concept to the routine use of single-particle ICP-MS UCT
13.4. RNDr. Oldřich Benada, CSc. Electron microscopy and X-ray microanalysis and their applications in nanoparticles research MBU CAS
20.4. RNDr. Jan Kratzer, Ph.D. Laser induced fluorescence and smart tricks of time-resolved spectrometry IAC CAS
27.4. RNDr. Jan Kratzer, Ph.D. Ambient mass spectrometry IAC CAS
4.5. RNDr. Vladimír Vrkoslav, Ph.D. Quantification and structural analysis of lipids and proteins by mass spectrometry IOCB CAS
11.5. Oral examination

IAC CAS – Institute of Analytical Chemistry, Czech Academy of Sciences
IOCB CAS - Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences
FS CU – Faculty of Science, Charles University
UCT – University of Chemistry and Technology Prague
MBU CAS – Institute of Microbiology, Czech Academy of Sciences
Literature -
Last update: RNDr. Jan Kratzer, Ph.D. (10.02.2022)

Handbook of High-resolution Spectroscopy. Wiley 2011
Laser Chemistry. Spectroscopy, Dynamics, and Applications. Wiley 2007
Surface-Enhanced Raman Spectroscopy. Analytical, Bioanalytical, and Life Science Application. Wiley 2010
Modern Spectroscopy. 4th Ed. Wiley 2004

Requirements to the exam -
Last update: RNDr. Jan Kratzer, Ph.D. (10.02.2022)

This subject will be finished by an oralexam. The exam will cover all topics lectured during the session.

Syllabus -
Last update: RNDr. Jan Kratzer, Ph.D. (10.02.2022)

Advanced spectrometric techniques used in atomic, molecular and mass spectrometry are discussed including their principles and applications. This year following techniques will be covered:

Raman spectroscopy, Raman optical activity (ROA), vibrational circular dichroism (VCD)

Single particle ICP-MS (sp-ICP-MS)

Speciation analysis by ICP-MS

Laser induced fluorescence (LIF) and two photon LIF (TALIF)

Optical emission spektrometry with high resolution in time and space

Electron microscopy (TEM, SEM)

X-ray microanalysis (EDS)

Ionization techniques for organic mass spectrometry (APCI, APPI, MALDI, ESI)

Ambient mass spectrometry


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