Advances in Separation Science - MC230P63

• Title: Advances in Separation Science
• Guaranteed by: Department of Analytical Chemistry (31-230)
• Faculty: Faculty of Science
• Actual: from 2011 to 2021
• Semester: summer
• E-Credits: 3
• Examination process: summer s.:written
• Hours per week, examination: summer s.:2/0, Ex [HT]
• Capacity: unlimited
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Level: specialized
• Note: enabled for web enrollment; priority enrollment if the course is part of the study plan
• Guarantor: doc. RNDr. Tomáš Křížek, Ph.D.
• Teacher(s): doc. RNDr. Tomáš Křížek, Ph.D.; RNDr. Anna Kubíčková, Ph.D.

Annotation

The course deals with modern separation techniques such as HPLC, UPLC, GC and CZE. Lectures should familiarize students with novelties in instrumentation and materials. Special attention will be paid to the recent applications of all these methods.
The course is intended for students with some basic knowledge of separation methods.

Last update: Nesměrák Karel, doc. RNDr., Ph.D. (05.06.2014)

Literature

R. Smith (Series Editor): Handbook of Analytical Separation, Volume 1 - 6, Elsevier, Amsterdam.

R. Kellner: Analytical chemistry : A modern approach to analytical science (2nd edition), Wiley-VCH, Weinheim, 2004.

D. C. Harris: Quantitative Chemical Analysis (7th edition), W. H. Freeman, New York, 2007.

Last update: Křížek Tomáš, doc. RNDr., Ph.D. (11.05.2017)

Requirements to the exam

The course will be finished by a written exam covering all lectured topics.

Last update: Křížek Tomáš, doc. RNDr., Ph.D. (07.03.2016)

Syllabus

HPLC - Modern stacionary phases. Monoliths. Miniaturization. Advances in detection techniques. Introduction to UPLC.

CZE - Dynamic and permanent capillary coatings. Enzyme assays. Lab on a chip.

GC - New approaches to the derivatization. Inverse GC. Multidimensional separation.

Advances in sample pretreatment.

Examples of recent applications.

Last update: Křížek Tomáš, doc. RNDr., Ph.D. (11.05.2017)