Advances in Separation Science - MC230P63

• Title: Advances in Separation Science
• Guaranteed by: Department of Analytical Chemistry (31-230)
• Faculty: Faculty of Science
• Actual: from 2011
• Semester: summer
• E-Credits: 3
• Examination process: summer s.:written
• Hours per week, examination: summer s.:2/0 Ex [hours/week]
• Capacity: unlimited
• Min. number of students: unlimited
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Level: specialized
• Note: enabled for web enrollment; priority enrollment if the course is part of the study plan
• Guarantor: doc. RNDr. Tomáš Křížek, Ph.D.

Annotation

Last update: doc. RNDr. Tomáš Křížek, Ph.D. (11.05.2017)

The course deals with modern separation techniques such as UPLC, SFC, GCxGC and CZE. It will familiarize students with novelties in instrumentation, materials and application approaches.
The course is intended for students who already have basic knowledge of separation methods. The lecture is taught in English for Erasmus students but is open to all students.
The course is organized in so called "flipped class" format in which students are given materials prior to each class to read through them at home. In the class clarifications are provided if necessary and students apply the acquired knowledge to solve problems with teacher's assistance. Active participation of students in the learning process and student-oriented teaching yield better understanding and better fixation of the acquired knowledge.

Literature

Last update: doc. RNDr. Tomáš Křížek, Ph.D. (11.05.2017)

R. Smith (Series Editor): Handbook of Analytical Separation, Volume 1 - 6, Elsevier, Amsterdam.
R. Kellner: Analytical chemistry : A modern approach to analytical science (2nd edition), Wiley-VCH, Weinheim, 2004.
D. C. Harris: Quantitative Chemical Analysis (7th edition), W. H. Freeman, New York, 2007.

Study materials on each topic will be provided by teachers during the course. 

Requirements to the exam

Last update: doc. RNDr. Tomáš Křížek, Ph.D. (02.05.2019)

66% attendance is required. The course will be finished by a written exam covering all lectured topics. 

Syllabus

Last update: doc. RNDr. Tomáš Křížek, Ph.D. (19.01.2018)

HPLC - Modern stacionary phases. Introduction to UPLC.
CZE - Sample stacking methods. Enzyme assays. 
GCxGC
SFC
HPLC-MS

Examples of recent applications.