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Last update: RNDr. Jana Sobotníková, Ph.D. (18.01.2018)
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Last update: RNDr. Jana Sobotníková, Ph.D. (18.01.2018)
1. http://www.natur.cuni.cz/~pcoufal, http://www.natur.cuni.cz/~suchan
2. Analytické separační metody, K. Štulík a kol., Karolinum, Praha, 2005.
3. Teoretické základy a separační principy kapilárních elekromigračních metod, V. Kašička, Chemické listy, 91 (1997) 320-329.
4. Analytická chemie, Z. Holzbecher, J. Churáček a kol., SNTL, Praha, 1987.
5. Instrumentální analýza, F. Čůta, M. Popl a kol., SNTL, Praha, 1986.
6. Plynová chromatografie I, II, III, E. Smolková, V. Pacáková, L. Feltl, K. Štulík, skripta PřF UK, Praha, 1976.
7. Vysokoúčinná kapalinová chromatografie, V. Pacáková, K. Štulík, skripta PřF UK, Praha, 1986.
8. Chromatography today, C.F. Poole, S.K. Poole, Elsevier, Amsterdam, 1991.
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Last update: RNDr. Jana Sobotníková, Ph.D. (18.01.2018)
Řádně odevzdaná zadaná semestrální práce je nezbytnou podmínkou pro zapsání se ke zkoušce. Zkouška ze separačních metod je písemná. Požadavky ke zkoušce jsou dány rozsahem přednášky. Pro úspěšné absolvování zkoušky je požadována minimálně 68% úspěšnost. |
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Last update: prof. RNDr. Pavel Coufal, Ph.D. (23.09.2020)
1. Introduction into separation methods, basic terms and theory of separation processes, classification of separation methods, interaction forces in separations processes.
2. Extraction, classification of extraction methods, extraction of inorganic, organic substances and ionic chelates, reextraction.
3. Solid phase extraction (SPE) and solid phase microextraction (SPME), principles, instrumentation and utilisation.
4. Supercritical fluid chromatography (SFE), supercritical medium, choice of supercritical fluid and modificator, types of analyte collection.
5. Affinity chromatography (AC), principles, affinity of ligands to macromolecules, binding of affinants to solid substrates, solid substrates and their selection, elution techniques.
6. Ion exchange chromatography (IEC), ionex types and their characterisation, application to inorganic and organic substances.
7. Gel permeation chromatography (GPC), molecular size exclusion effect, types of gels, separation of group of substances and desalination, fractionation and determination of relative molecular weight.
8. Gas chromatography (GC), adsorption and absorption, sorption isotherms, dissolution, rate and plate theories of GC, efficiency of GC, detectors, packed and capillary columns, injection techniques, stationary phases, qualitative and quantitative analysis, calibration procedures.
9. Chromatograph for high performance liquid chromatography (HPLC). Stationary and mobile phases, affinity, distribution and retardation. Thermodynamics and kinetics of separation. Retention quantities. General equation of chromatography. Zone broadening causes. Separation efficiency and resolution. Analytical information in chromatogram. Pumps, injection valves, columns and detectors. Stationary and mobile phases in LSC, LLC, IEC and GPC. Applications in HPLC.
10. Paper chromatography (PC). Thin layer chromatography (TLC). Development techniques. Detection and identification. Retardation factor. Densitometer. HPTLC. Applications in PC a TLC.
11. Electromigration methods. Electrophoretic migration and mobility. Electroosmotic flow and mobility. Electrical double layer and zeta-potencial. Velocity and temperature profile. Equipment for capillary zone electrophoresis (CZE). Separation mechanism in CZE. Electropherogram. Calculation of mobilities. Weak electrolytes. Sample injection. Detection. Analytical information in electropherogram. Applications in CZE.
References: http://www.natur.cuni.cz/~pcoufal http://www.natur.cuni.cz/~suchan Teoretické základy a separační principy kapilárních elekromigračních metod, V. Kašička, Chemické listy, 91 (1997) 320-329; Analytická chemie, Z. Holzbecher, J. Churáček a kol., SNTL, Praha, 1987; Instrumentální analýza, F. Čůta, M. Popl a kol., SNTL, Praha, 1986; Plynová chromatografie I, II, III, E. Smolková, V. Pacáková, L. Feltl, K. Štulík, skripta PřF UK, Praha, 1976; Vysokoúčinná kapalinová chromatografie, V. Pacáková, K. Štulík, skripta PřF UK, Praha, 1986; Chromatography today, C.F. Poole, S.K. Poole, Elsevier, Amsterdam, 1991; |