SubjectsSubjects(version: 901)
Course, academic year 2022/2023
  
Advanced Laboratory Course - MC230C13
Title: Pokročilé praktikum z analytické chemie (kata)
Czech title: Pokročilé praktikum z analytické chemie (kata)
Guaranteed by: Department of Analytical Chemistry (31-230)
Faculty: Faculty of Science
Actual: from 2020
Semester: both
E-Credits: 6
Hours per week, examination: 0/2 C [weeks/semester]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech, English
Note: enabled for web enrollment
you can enroll for the course in winter and in summer semester
Guarantor: RNDr. Jana Sobotníková, Ph.D.
Teacher(s): RNDr. Václav Červený, Ph.D.
RNDr. Hana Dejmková, Ph.D.
RNDr. Eliška Nováková, Ph.D.
prof. RNDr. František Opekar, CSc.
RNDr. Jana Sobotníková, Ph.D.
prof. RNDr. Vlastimil Vyskočil, Ph.D.
Opinion survey results   Examination dates   Schedule   
Annotation -
Last update: RNDr. Jana Sobotníková, Ph.D. (13.06.2022)
Students become familiar with conventional instrumental techniques of chemical analysis in this practical laboratory course. Taught analytical methods are divided into 3 basic groups: electrochemical, separation and spectrometric methods. The understanding of demonstrated principles of individual instrumental methods is emphasized. Students are led to handle raw experimental data, with their statistical evaluation and also presentation of calculated results. Throughout the course, strict compliance with GLP and safety regulations is required. Students should be able to work with common analytical instruments independently after finishing this course. The integral part of classification is continuous control of knowledge which is necessary to perform each task, classification of protocols and assessment of the laboratory work.

These laboratory exercises are organised in two weeks sessions while students cooperate in small groups. This laboratory course is organised in the summer and winter semesters. In the winter semester, the laboratory course is primarily intended for Erasmus students and for students of the 3rd year of KATA, who for serious reasons did not complete the course in the summer semester. In the winter semester, the course takes place at the end of November or in December and the capacity is limited.
Literature -
Last update: RNDr. Jana Sobotníková, Ph.D. (13.01.2022)

1. Task Instructions and Safety Regulations are available for download in the SIS.

2. K.Štulík a koletiv: Analytické separační metod, Univerzita Karlova v Praze, nakladatelství Karolinum 2004
3. Beran, P., Opekar, F., Štulík, K.: Instrumentální analytické metody, SPN Praha 1997
4. J. Churáček a kol., Analytická separace látek, SNTL, Praha 1990
5. Zýka a kol., Analytická příručka I. a II. díl, SNTL-Alfa, Praha 1988
6. K. Eckschlager, I. Horsák, Z. Kodejš, Vyhodnocování analytických výsledků a metod, SNTL, Praha 1980
7. M. Meloun, J. Militký, Statistické zpracování experimentálních dat, PLUS, Praha 1994

Requirements to the exam -
Last update: RNDr. Jana Sobotníková, Ph.D. (13.01.2022)

Credits are awarded after completing all the prescribed tasks in the course and the submission of all properly prepared protocols within 14 days after the end of the course.

Protocols are classified according to quality (results, statistical evaluation and processing) in the range from 1 (excellent) to 4 (unsatisfactory).

To be awarded the credits, the sum of marks of the classified protocols must not exceed the critical limit (25), which is determined at the beginning of the practical course.

 

 

Syllabus -
Last update: RNDr. Jana Sobotníková, Ph.D. (07.09.2021)

Following exercises are done:

1. Classical DC polarography
2. Karl Fischer titrations (KF)
3. Spectrophotometry - Determination of dissociation constant of an acid-base indicator
4. Determination of  Bi3+ and Cd2+ ions with AAS and K+ ion using Flame photometry
5. Gas chromatography (GC) - Qualitative and quantitative analysis
6. Determination of azodyes in a mixture using RP-HPLC method with spectrophotometric detection
7. Conductometry and conductometric titration
8. Capillary zone electrophoresis (CZE)

Entry requirements
Last update: RNDr. Jana Sobotníková, Ph.D. (13.01.2022)

Students should have knowledge about principles of electrochemical, separation and spectrometric analytical methods. Also evaluation of raw experimental data, their statistical evaluation and presentation of calculated results are necessary input requirements for attending this course. 

Registration requirements -
Last update: RNDr. Jana Sobotníková, Ph.D. (13.01.2022)

Students should have knowledge about principles of electrochemical, separation and spectrometric analytical methods. Also evaluation of raw experimental data, their statistical evaluation and presentation of calculated results are necessary input requirements for attending this course. 

 
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