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Course, academic year 2018/2019
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Practical course on proteomics - MB170C112
Title in English: Proteomické praktikum
Czech title: Proteomické praktikum
Guaranteed by: Department of Zoology (31-170)
Faculty: Faculty of Science
Actual: from 2014 to 2018
Semester: summer
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:0/1 C [weeks/semester]
Capacity: 12
Min. number of students: unlimited
State of the course: taught
Language: Czech
Additional information: http://email: petra@uhkt.cz, telefon: 224 965 865
Guarantor: doc. Mgr. Pavel Stopka, Ph.D.
Teacher(s): doc. RNDr. Jiří Petrák, Ph.D.
doc. Mgr. Pavel Stopka, Ph.D.
Pre-requisite : MB170P112
Annotation -
Last update: doc. RNDr. Jiří Petrák, Ph.D. (21.02.2013)
Please note, the lectures are given in Czech language. English version of the course can be requested in advance if there are at least 5 students.
To participate in this practical course students are required to complete course PROTEOMICS first. This one-week intensive practical course introduces participants into all aspects of typical proteomic analysis using 2D electrophoresis. In several successive steps participants will perform complete proteomic analysis starting with sample preparation and 2D electrophoretic analysis up to protein identification by mass spectrometry.

Literature - Czech
Last update: doc. RNDr. Jiří Petrák, Ph.D. (06.02.2013)

Výukové materiály i doporučenou literaturu naleznete na adrese: http://patofyziologie.lf1.cuni.cz/proteomika

Jedná se například o následující tituly:

Učebnice/Monografie:

R. K. Scopes: Protein purification. Prinicples and Practise, Springer Verlag, ISBN-10: 0387940723 | ISBN-13: 978-0387940724

J. Reinders, A. Sickman: Proteomics: Methods and Protocols (Methods in Molecular Biology). Humana Press ISBN-10: 1607611562 | ISBN-13: 978-1607611561

H. Rehm: Protein Biochemistry and Proteomics. Academic Press. ISBN-10: 012088545X | ISBN-13: 978-0120885459

Odborné články:

Gorg A et al. Current two-dimensional electrophoresis technology for proteomics, Proteomics, 2004, 4, 3665-3685

Requirements to the exam - Czech
Last update: doc. RNDr. Jiří Petrák, Ph.D. (21.02.2013)

Je udělován zápočet.

Syllabus -
Last update: SVATORA (26.05.2004)
  • Buffer and sample preparation. IPG strips rehydration and isoelectric focusing.
  • SDS PAGE, one- and two dimensional separations, gel staining - Coomassie blue, silver salts and fluorescent dyes .
  • Scanning and image analysis of gels a gel sets.
  • Selection and spot-picking, tryptic digestion and peptide extraction
  • Peptide mixture analysis by MS, protein identification. Database searching. Peptide fingerprinting and sequence tags. (MS and MS/MS analysis).

 
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