SubjectsSubjects(version: 908)
Course, academic year 2022/2023
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Annotation -
Last update: doc. RNDr. Jovana Sádlová, Ph.D. (02.09.2022)
Microscopic techniques is an exercise aimed to introduce students to light, fluorescence and electron microscopy.

The course consists of 3 blocks. The itinerary and all information you can find on Moodle: Přírodovědecká fakulta / ► Sekce biologie / ► Katedra parazitologie / ► MB160C45, with the password "Mikroskop-2022", active from 19th September 2022
Literature -
Last update: RNDr. Helena Kulíková (08.03.2019)

Study materials are in the Moodle on:

Přírodovědecká fakulta / ► Sekce biologie / ► Katedra parazitologie / ► kód předmětu

Requirements to the exam -
Last update: doc. RNDr. Jovana Sádlová, Ph.D. (24.10.2019)

Active participation on all three parts of the course is necessary. The credit is given after finalization of all the protocols in paper or electronical form (part 1 and parts 2-3, respectively).

Syllabus -
Last update: doc. RNDr. Jovana Sádlová, Ph.D. (26.08.2020)

1. Light microscopy (Sádlová, Vojtková, Majer, Šmídová, Bečvář, Kykalová)
Introduction to the basic theory of light microscopy and handling the light microscope. Students will prepare native and stained microscopic slides (Giemsa-Romanowski staining, aniline blue and native alizarin staining). The techniques will be demonstrated on protozoa with various taxonomic affiliation and helminths.

2. Special light and fluorescence microscopy 
(Sádlová, Vojtková, Majer, Šmídová, Bečvář, Kykalová)
Students will be introduced into the basics of the theory of contrasts in the light microscopy (dark field, phase contrast, DIC, Hoffman contrast), fluorescence and confocal microscopy. They will practically learn the work with dark field, phase contrast and fluorescence microscope. 

3. Electron microscopy (Sacherová, Juračka, Hyliš)
Students will be introduced into the theory of electron microscopy and preparation of specimens. They will have the chance to work practically with scanning and transmission electron microscope and prepare simple specimens. 


 
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