Microscopic Techniques for Teachers - MB160C42
Title: Mikroskopická technika pro učitele
Czech title: Mikroskopická technika pro učitele
Guaranteed by: Department of Parasitology (31-161)
Faculty: Faculty of Science
Actual: from 2024
Semester: winter
E-Credits: 2
Examination process: winter s.:
Hours per week, examination: winter s.:0/20, C [HS]
Capacity: 280
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Explanation: Zápis do paralelek jednotlivých bloků probíhá v prostředí Moodle, viz Anotace
Note: enabled for web enrollment
priority enrollment if the course is part of the study plan
Guarantor: doc. RNDr. Jovana Sádlová, Ph.D.
Teacher(s): prof. RNDr. Jana Albrechtová, Ph.D.
RNDr. Tomáš Bečvář, Ph.D.
Mgr. Aleš Benda, Ph.D.
Mgr. Iva Hammerbauerová
Mgr. Eva Hejduková, Ph.D.
Mgr. Anna Hošková
RNDr. Miroslav Hyliš, Ph.D.
Mgr. Kristýna Jelínková
RNDr. Petr Jan Juračka, Ph.D.
Mgr. Piotr Michal Jurkiewicz, Ph.D.
Mgr. Kateřina Kopalová, Ph.D.
Mgr. David Liebl, Ph.D.
Ing. Jiří Mikšátko, Ph.D.
Mgr. Marie Olšinová, Ph.D.
Ing. Dalibor Pánek, Ph.D.
Mgr. Matěj Pokorný
RNDr. Lenka Procházková, Ph.D.
Ing. Petra Prokšová, Ph.D.
Mgr. Adéla Přibylová, Ph.D.
doc. RNDr. Jovana Sádlová, Ph.D.
RNDr. Veronika Sacherová, Ph.D.
RNDr. Aleš Soukup, Ph.D.
Mgr. Kristýna Štěpánová
Mgr. Vojtěch Vajs
RNDr. Barbora Vojtková, Ph.D.
Incompatibility : MB160C45, MB160C45E
Is incompatible with: MB160C45
Opinion survey results   Examination dates   WS schedule   
Annotation -
A good mastery of the basics of microscopic technique is one of the basic practical didactic skills of a future biology teacher. Incorporating microscopy practical exercises into school teaching, or excursions to specialized microscopy facilities, is essential to motivate students to learn biology and to their understanding of the microscopic structure and function of organisms.

Microscopic Techniques” is a course aimed to introduce students to various types of microscopy (light microscopy, fluorescence and electron microscopy) and to the practical use of these techniques on model examples (counting of microscopic objects, digital image analysis). The course consists of 5 blocks. Active participation in all five parts of the course is necessary. The credit is given after the finalization of all the protocols in paper or electronic form (part 1 and parts 2-4, respectively). Pedagogues from several departments provide teaching, and it runs in different schoolrooms during the whole semester. The itinerary and all information you can find on Moodle pro výuku 2 / Přírodovědecká fakulta / ► Sekce biologie / ► Katedra parazitologie / ► MB160C45, with the password "Mikroskop-2024", active from 25th September 2024. Your questions address to sadlovaj@natur.cuni.cz.

Looking forward to your participation,
Jovana Sadlova and colleagues

Last update: SADLOVA/NATUR.CUNI.CZ (23.09.2024)
Literature -

Study materials are in the Moodle on:       

Přírodovědecká fakulta / ► Sekce biologie / ► Katedra parazitologie / ► MB160C45

Last update: SADLOVA/NATUR.CUNI.CZ (26.08.2024)
Requirements to the exam -

A condition for obtaining credit is the completion of all five blocks and submission of a report (block 1) or completion of an electronic test (blocks 2-5).

Last update: SADLOVA/NATUR.CUNI.CZ (26.08.2024)
Syllabus -

The course consists of five parts.

1. Light microscopy (Sádlová, Vojtková,  Bečvář, Hammerbauerová, Jelínková, Vajs)
Introduction to the basic theory of light microscopy and handling the light microscope. Students will prepare native and stained microscopic slides (Giemsa-Romanowski staining, aniline blue and native alizarin staining). The techniques will be demonstrated on protozoa with various taxonomic affiliation and helminths.

2. Special light and fluorescence microscopy (Sádlová, Vojtková, Bečvář, Hammerbauerová, Jelínková, Vajs)
Students will be introduced to the basics of the theory of contrasts in light microscopy (dark field, phase contrast, DIC, Hoffman contrast), fluorescence and confocal microscopy. They will practically learn the work with dark field, phase contrast and fluorescence microscope. 

3. Electron microscopy (Sacherová, Juračka, Hyliš)
Students will be introduced to the theory of electron microscopy and preparation of specimens. They will have a chance to work practically with scanning and transmission electron microscopes and prepare simple specimens. 

4. Introduction to the image analysis (Albrechtová, Soukup et al.)
In this section, students learn the basics of digitization and image processing and basic operations of image analysis. Practical exercises are conducted in the program ImageJ (National Institutes of Health, Bethesda, Maryland, USA, URL: http://imagej.nih.gov). More on the website of Block 4.

5. Advanced fluorescence and electron microscopy - excursion to the Service Laboratory of Imaging Methods BIOCEV (Aleš Benda et al.)

Students will be introduced to assets and basic principles of advanced microscopical methods (Super-Resolution, Fluorescence Lifetime Imaging Microscopy (FLIM), Correlative Light Electron Microscopy) with excursion to the service laboratory and presentation of individual microscopes (STED microscope, STORM and SIM microscope, MP microscope, FLIM microscope, SEM microscope).

 


Last update: SADLOVA/NATUR.CUNI.CZ (26.08.2024)