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Course, academic year 2024/2025
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Advances in developmental biology - MB151P117E
Title: Advances in developmental biology
Czech title: Pokroky ve vývojové biologii
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2024
Semester: winter
E-Credits: 4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unlimited
Min. number of students: 4
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: English
Note: enabled for web enrollment
priority enrollment if the course is part of the study plan
Guarantor: doc. RNDr. Ing. Vladimír Krylov, Ph.D.
Teacher(s): doc. Mgr. Robert Černý, Ph.D.
doc. RNDr. Ing. Vladimír Krylov, Ph.D.
Mgr. Marie Macůrková, Ph.D.
Mgr. Jan Mašek, Ph.D.
Mgr. Jiří Pergner, Ph.D.
Mgr. Vladimír Soukup, Ph.D.
Annotation -
The course put emphasis on the broadening the student´s knowledge regarding the advances in the developmental processes gained during bachelor study. Very important aspect is the leading the students toward critical thinking about published data and the ability to analyse the scientific text and present the data in front of the informed audience.
Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (27.05.2019)
Literature -

The course is based on the up to date reviews, primary scientific papers and world textbooks such as Gilbert et al., Developmental biology. 

Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (27.05.2019)
Requirements to the exam -

Requirements to the exam

Credits are given for the working out and presentation of essay focused on some selected topic regarding the recent research in the field of developmental biology. The exam is online writting test containig 20 single-option questions (A,B,C or D), two questions per lecture. One question = 5 points (100 points maximum in total). 

100 - 90 points = 1

89 - 80 points = 2
79 - 60 points = 3

59 - 0 points = 4 (not pass)

 

In the case you can´t work out the test at the faculty (foreign students, COVID issue etc), please let me know and I arrange the distant form for you. You will be invited to share your desktop and your face must be monitored by camera. If you don´t pass the writting test, the subsequent correction terms will be arranged via online or personal oral examination.  

Last update: Krylov Vladimír, doc. RNDr. Ing., Ph.D. (29.01.2021)
Syllabus -

1) From genotype to phenotype – Developmental information stored in DNA outside of protein-coding sequences - Jiri Pergner

The lecture will start with a reminder of textbook knowledge about non-protein coding information stored in DNA, particularly the structure of promoter sequences, miRNAs, and long non-coding RNAs. This knowledge will be broadened with recent information about their relationship to the Developmental processes. Next, the lecture will provide an overview of recent views on primary nuclear DNA sequences necessary for its organization in 3D space and the importance of such spatial distribution to the Developmental processes.

2) Gene regulatory networks and their features - Vladimir Soukup

Gene regulatory networks (GRN) during development, their features, logic, hierarchy, and spatial and temporal sequence. GRN components and mutual relationships, module examples, nods and their networking, inputs and outputs, cis-regulation. GRN during embryonic development and cell line specification in sea urchin model. GRN modifications and the evolution of body plans, evolutionary stability of Kernels, and plasticity of periphery parts. Evolution of new characters by modifying existing GRNs or co-opting GRNs for new features.

3) Early embryogenesis of non-vertebrates - Marie Macurkova

New views on the mosaic and regulatory development, asymmetry, the effect of the stochastic gene expression, and comparison of the early embryonic development regarding well-established and new non-vertebrate model organisms.

4) Jan Mašek - Advances in Early Vertebrate Embryogenesis

Embryonic stem cells, iPSCs, organoids, gastruloids, and in vitro embryos

5) Regulation of gene expression in organogenesis - Marie Macurkova

Signalling pathways regulating the development of selected organ systems, differences, and similarities among various animal groups, transcriptomics on the organ level.

6) Advances in Epithelial Developmental Biology - Robert Černý

Ectoderm-endoderm interactions in developing vertebrate head

7) Advances in Mesenchyme Developmental Biology - Robert Černý

Neural crest-derived mesenchyme as a prime example of developmental biology of mesenchymal cells

8) Limb development in vertebrates - Vladimír Krylov

Emergence of the limb bud, polarization of the developing limbs, the role of hox genes and their gene products including downstream signalling pathways. Failures during limb development, evolutionary comparison of the limb development among vertebrates.

9) Embryonic development of the organs basis in vertebrates - Vladimír Krylov

Embryonic development of the heart, digestive and respiratory tract, primary gonads and kidney. Evolutionary comparison.

10) Regeneration - Vladimir Soukup

Regeneration vs. healing. Examples of regenerating tissues in the animal kingdom, regeneration in vertebrates. Tailed amphibians as masters of regeneration. Tail and limb regeneration. Dedifferentiation, transdifferentiation and cellular processes during regeneration. Regeneration as a repetition of development?)



 The course is taught with the support of the project reg. number CZ.02.2.69/0.0/0.0/16_015/0002362

Last update: Mašek Jan, Mgr., Ph.D. (29.09.2023)
Entry requirements - Czech

Zvládnout výuku v anglickém jazyce.

Last update: Šebková Nataša, RNDr., Ph.D. (12.09.2019)
 
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