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Course, academic year 2022/2023
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Advances in developmental biology - MB151P117E
Title: Advances in developmental biology
Czech title: Pokroky ve vývojové biologii
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2019
Semester: winter
E-Credits: 4
Examination process: winter s.:
Hours per week, examination: winter s.:2/1, C+Ex [HT]
Capacity: unlimited
Min. number of students: 4
For 4EU+ students: no
Virtual mobility / capacity: no
State of the course: taught
Language: English
Note: enabled for web enrollment
Guarantor: doc. RNDr. Ing. Vladimír Krylov, Ph.D.
Teacher(s): doc. Mgr. Robert Černý, Ph.D.
doc. RNDr. Ing. Vladimír Krylov, Ph.D.
Mgr. Marie Macůrková, Ph.D.
Mgr. Jiří Pergner, Ph.D.
Mgr. Vladimír Soukup, Ph.D.
Annotation -
Last update: doc. RNDr. Ing. Vladimír Krylov, Ph.D. (27.05.2019)
The course put emphasis on the broadening the student´s knowledge regarding the advances in the developmental processes gained during bachelor study. Very important aspect is the leading the students toward critical thinking about published data and the ability to analyse the scientific text and present the data in front of the informed audience.
Literature -
Last update: doc. RNDr. Ing. Vladimír Krylov, Ph.D. (27.05.2019)

The course is based on the up to date reviews, primary scientific papers and world textbooks such as Gilbert et al., Developmental biology. 

Requirements to the exam -
Last update: doc. RNDr. Ing. Vladimír Krylov, Ph.D. (29.01.2021)

Requirements to the exam

Credits are given for the working out and presentation of essay focused on some selected topic regarding the recent research in the field of developmental biology. The exam is online writting test containig 20 single-option questions (A,B,C or D), two questions per lecture. One question = 5 points (100 points maximum in total). 

100 - 90 points = 1

89 - 80 points = 2
79 - 60 points = 3

59 - 0 points = 4 (not pass)


In the case you can´t work out the test at the faculty (foreign students, COVID issue etc), please let me know and I arrange the distant form for you. You will be invited to share your desktop and your face must be monitored by camera. If you don´t pass the writting test, the subsequent correction terms will be arranged via online or personal oral examination.  

Syllabus -
Last update: doc. RNDr. Ing. Vladimír Krylov, Ph.D. (12.09.2023)

1) From genotype to phenotype - changes in gene expression regarding developmental processes - Jiri Pergner

Changes in the large-effect gene regulation leading to the large phenotypic changes (i.e. mutations in enhancers; mutations leading to the formation of silencers) vs. small phenotypic changes caused by the changes in gene expression of many genes. Shadow enhancers as a tool to ensure the robustness of the transcription regulation. Presentation of non-standard model organisms employed in the evo-devo studies.

2) Gene regulatory networks and their features - Vladimir Soukup

Gene regulatory networks (GRN) during development, their features, logic, hierarchy and spatial and temporal sequence. GRN components and mutual relationships, module examples, nods and their networking, inputs and outputs, cis regulation. GRN during embryonic development and cell line specification in sea urchin model. GRN modifications and the evolution of body plans, evolutionary stability of Kernels and plasticity of periphery parts. Evolution of new characters by modifying existing GRNs or co-opting GRNs for new features.

3) Role of the genome 3D structure and “junk DNA” in developmental processes - Jiří Pergner

Up to date perspective on the genome 3D structure regarding the regulation of transcription - Topologically associated domains (TADs). TADs features, their changes in the evolution and during ontogenesis. Non-coding RNAs regulating the gene expression

4) Early embryogenesis of non-vertebrates - Marie Macurkova

New views on the mosaic and regulatory development, asymetry, the effect of the stochastic gene expression, comparison of the early embryonic development regarding well established and new non-vertebrate model organisms.

5) Regulation of gene expresion in organogenesis - Marie Macurkova

Signalling pathways regulating the development of selected organ systems, differences and similarities among various animal groups, transcriptomics on the organ level.

6) Advances in Epithelial Developmental Biology - Robert Černý

Ectoderm-endoderm interactions in developing vertebrate head

7) Limb development in vertebrates - Vladimír Krylov

Emergence of the limb bud, polarization of the developing limbs, the role of hox genes and their gene products including downstream signalling pathways. Failures during limb development, evolutionary comparison of the limb development among vertebrates.

8) Regeneration - Vladimir Soukup

Regeneration vs. healing. Examples of regenerating tissues in the animal kingdom, regeneration in vertebrates. Tailed amphibians as masters of regeneration. Tail and limb regeneration. Dedifferentiation, transdifferentiation and cellular processes during regeneration. Regeneration as a repetition of development?)

9) Embryonic development of the organs basis in vertebrates - Vladimír Krylov

Embryonic development of the heart, digestive and respiratory tract, primary gonads and kidney. Evolutionary comparison.

10) Advances in Mesenchyme Developmental Biology - Robert Černý

Neural crest-derived mesenchyme as the prime example of developmental biology of mesenchymal cells

 The course is taught with the support of the project reg. number CZ.02.2.69/0.0/0.0/16_015/0002362

Entry requirements - Czech
Last update: RNDr. Nataša Šebková, Ph.D. (12.09.2019)

Zvládnout výuku v anglickém jazyce.

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