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Fluorescent histology - practical course - MB151C05E

Title:	Fluorescent histology - practical course
Czech title:	Praktikum z fluorescenční histologie
Guaranteed by:	Department of Cell Biology (31-151)
Faculty:	Faculty of Science
Actual:	from 2023 to 2023
Semester:	winter
E-Credits:	3
Examination process:	winter s.:
Hours per week, examination:	winter s.:0/1, C [TS]
Capacity:	20
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	English
Explanation:	Předmět je určen pouze pro magisterské studenty, případně pro studentypostgraduálního studia
Note:	enabled for web enrollment

Guarantor:	prof. RNDr. Jan Černý, Ph.D.

Opinion survey results Examination dates WS schedule

Annotation -

Last update: RNDr. Nataša Šebková, Ph.D. (24.10.2019)

Fluorescence histological methods have revolutionized the study of developmental processes. Practical exercises will enable to understand principles of fluorescence histological techniques, preparation of preparations and their microscopic processing. Genetically modified model organisms of evolutionary biology - nematode, frog claw and mouse expressing fluorescent proteins will be used. During the practical exercise, microscopes acquired from the OP VVV project - light sheet microscope, virtual-slide microscope and life-cell imager will be used intensively. The main aim of the practical exercise is to acquaint students with modern approaches to the study of developmental processes that do not resign to the complexity of cellular arrangements.

Literature -

Last update: RNDr. Nataša Šebková, Ph.D. (21.02.2018)

Histology: A Text and Atlas, with Correlated Cell and Molecular Biology, 6th Edition 6th Edition, Michael H. Ross a Wojciech Pawlina MD ISBN-13: 978-0781772006, ISBN-10: 0781772001

Requirements to the exam -

Last update: RNDr. Nataša Šebková, Ph.D. (25.10.2019)

The theoretical and practical knowledge gained in practical exercises will be tested.

Syllabus -

Last update: RNDr. Nataša Šebková, Ph.D. (24.10.2019)

1. Theory of fluorescence techniques

2. Preparation of cross-sectional microscopic preparations from transgenic (or injected fluorescent probes) models

3. Use of fluorescent probes and antibodies

4. Study of cellular processes in vivo in situ

5. Preparation of whole mount histological preparations

Advanced microscopic techniques including teaching practical microscopy and data analysis

Entry requirements - Czech

Last update: RNDr. Nataša Šebková, Ph.D. (21.02.2018)

Zvládnout výuku v anglickém jazyce.