Experimental work with mammalian oocytes and early embryos - MB151C04
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The goal of the practice course is obtaining a basic experience with experimental techniques of isolation and in vitro culture of mammalian oocytes and embryos. A basic introduction into the live oocyte and embryo imaging will be provided. The primary language of the course is Czech but when at least one student request course will be in English.
Last update: Šebková Nataša, RNDr., Ph.D. (04.09.2019)
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A laboratory practice course – a experimental protocol will be provided Last update: Šebková Nataša, RNDr., Ph.D. (04.09.2019)
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active participation on the cource, preparation of own experimental reports Last update: Šebková Nataša, RNDr., Ph.D. (04.09.2019)
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Mouse hormonal stimulation for obtaining prophase I oocytes Prophase I oocyte isolation In vitro culture of oocytes into metaphase II Visualization of chromosomes and spindles using fluorogenic drugs SiR-DNA and SiR-tubulin and live imaging Computer image analysis of spindle formation and chromosome segregation (Hormonal stimulation of female mice and breeding with males, isolation of one-cell stage embryos and in vitro culture to two-cell stage embryos) The course is taught with the support of the project reg. number CZ.02.2.69/0.0/0.0/16_015/0002362Last update: Rubešová Jana, RNDr., Ph.D. (14.09.2019)
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